Purification of IgD from human serum - A novel application of recombinant M. catarrhalis IgD-binding protein (MID).

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Abstract

Moraxella catarrhalis IgD-binding protein (MID) is a multimeric outer membrane protein belonging to the family of autotransporters. The IgD-binding domain of MID is located between amino acids MID 962-1200 and binds to amino acids 198-224 of the IgD C(H)1 region. In the present study, we describe a method to purify IgD from serum with high levels of IgD using a two-step affinity chromatography process. The first step involves depletion of MID-specific antibodies of all classes from serum using the non-IgD-binding fragment MID1000-1200. This step is followed by selective capture of IgD with MID962-1200. Furthermore, we demonstrate that the eluted IgD is pure, intact and functional for use in downstream applications. Our approach reduces the non-specificity commonly associated with lectin-based IgD purification regimes that rely on glycosylation of the IgD molecule. (c) 2006 Elsevier B.V. All rights reserved.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area

Keywords

  • purification, immunoglobulin D, Moraxella catarrhalis, MID
Original languageEnglish
Pages (from-to)31-37
JournalJournal of Immunological Methods
Volume317
Publication statusPublished - 2006
Publication categoryResearch
Peer-reviewedYes