Purification of the transforming growth factor-beta (TGF-beta) binding proteoglycan betaglycan

Research output: Contribution to journalArticle

Abstract

We report the purification of betaglycan, a low-abundance membrane proteoglycan with high affinity for transforming growth factor-beta (TGF-beta). Betaglycan solubilized from rat embryo membrane preparations was purified to near-homogeneity by sequential chromatography through DEAE-Trisacryl, wheat germ lectin-Sepharose, and TGF-beta 1-agarose. Purified betaglycan has properties similar to betaglycan affinity-labeled in intact cells: it binds TGF-beta 1 and TGF-beta 2 with KD approximately 0.2 nM, contains heparan sulfate and chondroitin sulfate glycosaminoglycan (GAG) chains and N-linked glycans attached to a 110-kDa core protein, and can spontaneously associate with phosphatidylcholine liposomes. The betaglycan core obtained by enzymatic removal of the GAG chains has high affinity for TGF-beta and associates with artificial liposomes, indicating that the core protein binds TGF-beta and anchors to membranes independently of the GAG chains present on the native protein or of any ancillary protein.

Details

Authors
External organisations
  • External Organization - Unknown
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medicinal Chemistry

Keywords

  • Animals Binding, Affinity Chromatography, Ion Exchange Humans Liposomes/metabolism Membrane Proteins/*isolation & purification/metabolism Proteoglycans/*isolation & purification/metabolism Rats *Receptors, Competitive Cattle Chromatography, Transforming Growth Factor beta Transforming Growth Factor beta/*metabolism
Original languageEnglish
Pages (from-to)23282-23287
JournalJournal of Biological Chemistry
Volume266
Issue number34
Publication statusPublished - 1991
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Experimental Clinical Chemistry (013016010)