Quality of up to 35 years old archival breast cancer tissue in paraffinblocks for estrogen receptor evaluation

Research output: Contribution to journalPublished meeting abstract

Abstract

Objective: Estrogen receptor (ER) positive breast cancer (BC) can have
an insidious course with disease-relapse decades after primary surgery.
New analysis performed on archived formalin-fixed paraffin-embedded(FFPE) tissue are important for disease-management in late BC-relapse
and an important tool in BC-research. However, although loss of immunoreactivity
in tissue slides after sectioning has been shown, little is
known of the preservation of biomarker-expression in FFPE tumourblocks.
We aim to investigate the quality of immunohistochemical
(IHC) ER-evaluation in FFPE-tissue over time (1978–2000).
Method: Tissue-microarrays from a Swedish multicenter cohort of 728
patients with contralateral BC was used for ER IHC-evaluation. BC was
studied in three periods (1958–1985, 1986–1993, 1994–2000), and retrospective
ER IHC-data was correlated to corresponding prospective ER
cytosol-analysis performed on fresh BC-tissue.
Results: The concordance between the original ER cytosol-analysis and
the new IHC was substantial (1978–1985: 82 %, (117/142), Kappa 0.52.
1986–1993: 91 %, (194/213), Kappa 0.72. 1994–2000: 86 %, (187/218),
Kappa 0.61). Discrepancies were mostly found for tumours with ERvalues
close to cutoff for one or both of the methods.
Conclusion: FFPE BC-tissue from the late 70s to millennium shows
preserved ER-antigenicity up to 35 years later.

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Subject classification (UKÄ) – MANDATORY

  • Cancer and Oncology
Original languageEnglish
Article numberPS-01-016
Pages (from-to)54
Number of pages1
JournalVirchows Archiv
Volume471
Issue numberSuppl. 1
Publication statusPublished - 2017 Sep 2
Publication categoryResearch
Peer-reviewedYes
Event29th European Congress of Pathology - RAI, Amsterdam, Netherlands
Duration: 2017 Sep 2 → …
Conference number: 29

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Anna Ehinger, Mårten Fernö, Sara Alkner, Dorthe Grabau, Pär-Ola Bendahl, Lisa Ryden, Per Malmström, Carina Forsare, Anna-Karin Falck, Sara Baker & Kristina Lövgren

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Project: Dissertation

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Ehinger, A. (Participant)
2017 Sep 22017 Sep 6

Activity: Participating in or organising an eventParticipation in conference

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