Quantification of Human Kallikrein-2 in Clinical Samples by Selected Reaction Monitoring

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Quantification of Human Kallikrein-2 in Clinical Samples by Selected Reaction Monitoring. / Végvári, Ákos; Sjödin, Karin; Rezeli, Melinda; Marko-Varga, György.

In: Journal of Proteome Research, Vol. 12, No. 10, 2013, p. 4612-4616.

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TY - JOUR

T1 - Quantification of Human Kallikrein-2 in Clinical Samples by Selected Reaction Monitoring

AU - Végvári, Ákos

AU - Sjödin, Karin

AU - Rezeli, Melinda

AU - Marko-Varga, György

PY - 2013

Y1 - 2013

N2 - Recently, the number of mass spectrometry-based quantification assays has been increased, partly due to the global efforts of chromosome-centric human proteome project (C-HPP). Our goal at the Chromosome 19 Consortium is to provide novel selected reaction monitoring (SRM) assays of proteins coded on chromosome 19. We have selected the two most useful signature peptides (NSQVWLGR and HNLFEPEDTGQR) of human kallikrein-2 (hK2 – NX_P20151) and developed an SRM assay. Details about the analytical parameters, including multiple transitions by peptides are presented. The endogenous levels of hK2 were determined in clinical samples (n=35). The limit of quantification was also estimated by spiking heavy isotope labeled peptides into seminal plasma samples at various concentrations (LOQ ≈ 29 ng/mL).

AB - Recently, the number of mass spectrometry-based quantification assays has been increased, partly due to the global efforts of chromosome-centric human proteome project (C-HPP). Our goal at the Chromosome 19 Consortium is to provide novel selected reaction monitoring (SRM) assays of proteins coded on chromosome 19. We have selected the two most useful signature peptides (NSQVWLGR and HNLFEPEDTGQR) of human kallikrein-2 (hK2 – NX_P20151) and developed an SRM assay. Details about the analytical parameters, including multiple transitions by peptides are presented. The endogenous levels of hK2 were determined in clinical samples (n=35). The limit of quantification was also estimated by spiking heavy isotope labeled peptides into seminal plasma samples at various concentrations (LOQ ≈ 29 ng/mL).

KW - Chromosome 19

KW - Human kallikrein-2

KW - Selected reaction monitoring

KW - Mass spectrometry

U2 - 10.1021/pr400423k

DO - 10.1021/pr400423k

M3 - Article

VL - 12

SP - 4612

EP - 4616

JO - Journal of Proteome Research

T2 - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 10

ER -