Quantification of Membrane Proteins Using Nonspecific Protease Digestions
Research output: Contribution to journal › Article
We present a mass spectrometry-based method for the identification and quantification of membrane proteins using the low-specificity protease Proteinase K, at very high pH, to digest proteins isolated by a modified SDS-PAGE protocol. The resulting peptides are modified with a fragmentation-directing isotope labeled tag. We apply the method to quantify differences in membrane protein expression of Bacillus subtilis grown in the presence or absence of glucose.
|Research areas and keywords||
Subject classification (UKÄ) – MANDATORY
|Journal||Journal of Proteome Research|
|Publication status||Published - 2009|