Quantitative analysis of rat Ig (sub) classes binding to cell surface antigens

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Abstract

An indirect 125I-labeled protein A assay for detection of cell surface-bound rat immunoglobulins is presented. The assay is quantitative and rapid and detects as little as 1 ng of cell surface-bound Ig. It discriminates between antibodies belonging to different IgG subclasses, IgM and IgA. We describe the production and specificity control of the reagents used and show that the test can be used for quantitative analysis. A large number of sera from untreated rats are tested to evaluate the frequency of falsely positive responses and variation due to age, sex and strain of rat. With this test it is relatively easy to quantitate the binding of classes and subclasses of rat immunoglobulins in a small volume (6 μl) of untreated serum.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area

Keywords

  • protein A, cell surface antigens, rat Ig (sub)classes, binding test, monoclonal Ig
Original languageEnglish
Pages (from-to)179-191
JournalJournal of Immunological Methods
Volume55
Issue number2
Publication statusPublished - 1982
Publication categoryResearch
Peer-reviewedYes