Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells

Research output: Contribution to journalArticle


SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We identified 5587 proteins, 366 of them with differential abundance. Differentiated cells expressed proteins related to neuronal development, and, undifferentiated cells expressed proteins involved in cell proliferation. Interactive network covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases processes and regulation of mitogen-activated protein kinase-related signaling pathways; key proteins involved in those processes might be explored as markers for neuronal differentiation.


  • Jimmy Rodriguez Murillo
  • Livia Goto-Silva
  • Aniel Sánchez
  • Fábio C.S. Nogueira
  • Gilberto B. Domont
  • Magno Junqueira
External organisations
  • Federal University of Rio de Janeiro
  • Skåne University Hospital
  • D'Or Institute for Research and Education
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology
  • Neurosciences


  • iTRAQ-based proteomics, Neuronal differentiation, Phosphoproteomics, SH-SY5Y cells
Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalEuPA Open Proteomics
Publication statusPublished - 2017 Sep 1
Publication categoryResearch