Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells

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Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells. / Murillo, Jimmy Rodriguez; Goto-Silva, Livia; Sánchez, Aniel; Nogueira, Fábio C.S.; Domont, Gilberto B.; Junqueira, Magno.

In: EuPA Open Proteomics, Vol. 16, 01.09.2017, p. 1-11.

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Murillo, Jimmy Rodriguez ; Goto-Silva, Livia ; Sánchez, Aniel ; Nogueira, Fábio C.S. ; Domont, Gilberto B. ; Junqueira, Magno. / Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells. In: EuPA Open Proteomics. 2017 ; Vol. 16. pp. 1-11.

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TY - JOUR

T1 - Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells

AU - Murillo, Jimmy Rodriguez

AU - Goto-Silva, Livia

AU - Sánchez, Aniel

AU - Nogueira, Fábio C.S.

AU - Domont, Gilberto B.

AU - Junqueira, Magno

PY - 2017/9/1

Y1 - 2017/9/1

N2 - SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We identified 5587 proteins, 366 of them with differential abundance. Differentiated cells expressed proteins related to neuronal development, and, undifferentiated cells expressed proteins involved in cell proliferation. Interactive network covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases processes and regulation of mitogen-activated protein kinase-related signaling pathways; key proteins involved in those processes might be explored as markers for neuronal differentiation.

AB - SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We identified 5587 proteins, 366 of them with differential abundance. Differentiated cells expressed proteins related to neuronal development, and, undifferentiated cells expressed proteins involved in cell proliferation. Interactive network covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases processes and regulation of mitogen-activated protein kinase-related signaling pathways; key proteins involved in those processes might be explored as markers for neuronal differentiation.

KW - iTRAQ-based proteomics

KW - Neuronal differentiation

KW - Phosphoproteomics

KW - SH-SY5Y cells

U2 - 10.1016/j.euprot.2017.06.001

DO - 10.1016/j.euprot.2017.06.001

M3 - Article

C2 - 29900121

AN - SCOPUS:85023609384

VL - 16

SP - 1

EP - 11

JO - EuPA Open Proteomics

JF - EuPA Open Proteomics

SN - 2212-9685

ER -