Rapid fluorometric screening of antibiotics in seafood

Research output: Contribution to journalArticle

Abstract

Simple and rapid fluorometric screening methods have been developed based on the competitive binding between the target and an intercalating fluorophore dye to double-stranded-DNA (dsDNA). In this study, the long-wavelength fluorescente dye TOTO-3 was employed as the indicator. Compounds that interact with dsDNA will affect the binding of TOTO-3 to the nucleic acid thereby changing the fluorescence intensity. The analyte concentration is indirectly determined by the decrease in fluorescence intensity. A fiber optic fluorescence screening system was developed for rapid and convenient sample processing. Lambda DNA (48.5kb) was chosen as a suitable sensing nucleic acid material. Detection of sulfathiazole and chloramphenicol in shrimps using this method was studied in the range of 0.5-25ng mL(-1) of sulfathiazole and of 1-50ng mL(-1) of chloramphenicol. Detection limits of 0.5ng mL(-1) of sulfathiazole and 1ng mL(-1) of chloramphenicol were achieved. This approach is useful as a routine test in the monitoring of antibiotics in the environment or aquaculture products. The easy operation and the rapid and sensitive detection make this a potential high-throughput screening method.

Details

Authors
  • Y Liu
  • Bengt Danielsson
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology

Keywords

  • fluorometric screening, screening, DNA-based drug, sulfathiazole, chloramphenicol, seafood contamination
Original languageEnglish
Pages (from-to)133-137
JournalMikrochimica Acta
Volume153
Issue number3-4
Publication statusPublished - 2006
Publication categoryResearch
Peer-reviewedYes