Rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted respiratory distress syndrome with high sensitivity

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@article{06bf95182e4e4befb1e40e9bb5c7443d,
title = "Rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted respiratory distress syndrome with high sensitivity",
abstract = "Aim: Respiratory distress syndrome (RDS) is a major cause of mortality and morbidity in premature infants. By the time symptoms appear, it may already be too late to prevent a severe course, with bronchopulmonary dysplasia or mortality. We aimed to develop a rapid test of lung maturity for targeting surfactant supplementation. Methods: Concentrations of the most surface-active lung phospholipid dipalmitoylphosphatidylcholine and sphingomyelin in gastric aspirates from premature infants were measured by mass spectrometry and expressed as the lecithin/sphingomyelin ratio (L/S). The same aspirates were analysed with mid-infrared spectroscopy. Subsequently, L/S was measured in gastric aspirates and oropharyngeal secretions from another group of premature infants using spectroscopy and the results were compared with RDS development. The 10-minute analysis required 10 μL of aspirate. Results: An L/S algorithm was developed based on 89 aspirates. Subsequently, gastric aspirates were sampled in 136 infants of 24-31 weeks of gestation and 61 (45{\%}) developed RDS. The cut-off value of L/S was 2.2, sensitivity was 92{\%}, and specificity was 73{\%}. In 59 cases, the oropharyngeal secretions had less valid L/S than gastric aspirate results. Conclusion: Our rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted RDS with high sensitivity.",
keywords = "Gastric aspirate, Lung surfactant, Mid-infrared spectroscopy, Prematurity, Respiratory distress syndrome",
author = "Henrik Verder and Christian Heiring and Howard Clark and David Sweet and Jessen, {Torben E.} and Finn Ebbesen and Bj{\"o}rklund, {Lars J.} and Bengt Andreasson and Lars Bender and Aksel Bertelsen and Marianne Dahl and Christian Eschen and Jesper Fenger-Gr{\o}n and Hoffmann, {Stine F.} and Agnar H{\"o}skuldsson and Maria Bruusgaard-Mouritsen and Fredrik Lundberg and Postle, {Anthony D.} and Peter Schousboe and Peter Schmidt and Hristo Stanchev and Lars S{\o}rensen",
year = "2017",
doi = "10.1111/apa.13683",
language = "English",
volume = "106",
pages = "430--437",
journal = "Acta P{\ae}diatrica",
issn = "1651-2227",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "3",

}