Rapid tracing of resistance plasmids in a nosocomial outbreak using optical DNA mapping

Research output: Contribution to journalArticle

Abstract

Resistance to life-saving antibiotics increases rapidly worldwide, and multiresistant bacteria have become a global threat to human health. Presently, the most serious threat is the increasing spread of Enterobacteriaceae carrying genes coding for extended spectrum β-lactamases (ESBL) and carbapenemases on highly mobile plasmids. We here demonstrate how optical DNA maps of single plasmids can be used as fingerprints to trace plasmids, for example, during resistance outbreaks. We use the assay to demonstrate a potential transmission route of an ESBL-carrying plasmid between bacterial strains/species and between patients, during a polyclonal outbreak at a neonatal ward at Sahlgrenska University Hospital (Gothenburg, Sweden). Our results demonstrate that optical DNA mapping is an easy and rapid method for detecting the spread of plasmids mediating resistance. With the increasing prevalence of multiresistant bacteria, diagnostic tools that can aid in solving ongoing routes of transmission, in particular in hospital settings, will be of paramount importance.

Details

Authors
Organisations
External organisations
  • Chalmers University of Technology
  • University of Gothenburg
  • Lund University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medicinal Chemistry
  • Infectious Medicine

Keywords

  • antibiotic resistance, nosocomial outbreak, optical DNA mapping, plasmids
Original languageEnglish
Pages (from-to)322-328
Number of pages7
JournalACS Infectious Diseases
Volume2
Issue number5
Publication statusPublished - 2016 May 13
Publication categoryResearch
Peer-reviewedYes