Rare allelic forms of PRDM9 associated with childhood leukemogenesis

Research output: Contribution to journalArticle

Abstract

One of the most rapidly evolving genes in humans, PRDM9, is a key determinant of the distribution of meiotic recombination events. Mutations in this meiotic-specific gene have previously been associated with male infertility in humans and recent studies suggest that PRDM9 may be involved in pathological genomic rearrangements. In studying genomes from families with children affected by B-cell precursor acute lymphoblastic leukemia (B-ALL), we characterized meiotic recombination patterns within a family with two siblings having hyperdiploid childhood B-ALL and observed unusual localization of maternal recombination events. The mother of the family carries a rare PRDM9 allele, potentially explaining the unusual patterns found. From exomes sequenced in 44 additional parents of children affected with B-ALL, we discovered a substantial and significant excess of rare allelic forms of PRDM9. The rare PRDM9 alleles are transmitted to the affected children in half the cases; nonetheless there remains a significant excess of rare alleles among patients relative to controls. We successfully replicated this latter observation in an independent cohort of 50 children with B-ALL, where we found an excess of rare PRDM9 alleles in aneuploid and infant B-ALL patients. PRDM9 variability in humans is thought to influence genomic instability, and these data support a potential role for PRDM9 variation in risk of acquiring aneuploidies or genomic rearrangements associated with childhood leukemogenesis.

Details

Authors
  • Julie Hussin
  • Daniel Sinnett
  • Ferran Casals
  • Youssef Idaghdour
  • Vanessa Bruat
  • Virginie Saillour
  • Jasmine Healy
  • Jean-Christophe Grenier
  • Thibault de Malliard
  • Stephan Busche
  • Jean-François Spinella
  • Mathieu Larivière
  • Greg Gibson
  • Linda Holmfeldt
  • Jing Ma
  • Lei Wei
  • Jinghui Zhang
  • Gregor Andelfinger
  • James R Downing
  • Charles G Mullighan
  • Philip Awadalla
External organisations
  • University of Montreal, Canada
  • St Jude Children´s Research Hospital, Memphis
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medical Genetics
  • Cancer and Oncology

Keywords

  • Adolescent, Alleles, Child, Child, Preschool, Cohort Studies, Crossing Over, Genetic, Exome, Female, Gene Frequency, Gene Rearrangement, Genomic Instability, Histone-Lysine N-Methyltransferase/genetics, Humans, Infant, Leukemia, Biphenotypic, Acute/genetics, Male, Meiosis, Microarray Analysis, Mutation, Pedigree, Polymorphism, Single Nucleotide, Recombination, Genetic, Sequence Analysis, DNA, Translocation, Genetic
Original languageEnglish
Pages (from-to)419-30
Number of pages12
JournalGenome Research
Volume23
Issue number3
Publication statusPublished - 2013 Mar
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes