Rare allelic forms of PRDM9 associated with childhood leukemogenesis

Research output: Contribution to journalArticle


title = "Rare allelic forms of PRDM9 associated with childhood leukemogenesis",
abstract = "One of the most rapidly evolving genes in humans, PRDM9, is a key determinant of the distribution of meiotic recombination events. Mutations in this meiotic-specific gene have previously been associated with male infertility in humans and recent studies suggest that PRDM9 may be involved in pathological genomic rearrangements. In studying genomes from families with children affected by B-cell precursor acute lymphoblastic leukemia (B-ALL), we characterized meiotic recombination patterns within a family with two siblings having hyperdiploid childhood B-ALL and observed unusual localization of maternal recombination events. The mother of the family carries a rare PRDM9 allele, potentially explaining the unusual patterns found. From exomes sequenced in 44 additional parents of children affected with B-ALL, we discovered a substantial and significant excess of rare allelic forms of PRDM9. The rare PRDM9 alleles are transmitted to the affected children in half the cases; nonetheless there remains a significant excess of rare alleles among patients relative to controls. We successfully replicated this latter observation in an independent cohort of 50 children with B-ALL, where we found an excess of rare PRDM9 alleles in aneuploid and infant B-ALL patients. PRDM9 variability in humans is thought to influence genomic instability, and these data support a potential role for PRDM9 variation in risk of acquiring aneuploidies or genomic rearrangements associated with childhood leukemogenesis.",
keywords = "Adolescent, Alleles, Child, Child, Preschool, Cohort Studies, Crossing Over, Genetic, Exome, Female, Gene Frequency, Gene Rearrangement, Genomic Instability, Histone-Lysine N-Methyltransferase/genetics, Humans, Infant, Leukemia, Biphenotypic, Acute/genetics, Male, Meiosis, Microarray Analysis, Mutation, Pedigree, Polymorphism, Single Nucleotide, Recombination, Genetic, Sequence Analysis, DNA, Translocation, Genetic",
author = "Julie Hussin and Daniel Sinnett and Ferran Casals and Youssef Idaghdour and Vanessa Bruat and Virginie Saillour and Jasmine Healy and Jean-Christophe Grenier and {de Malliard}, Thibault and Stephan Busche and Jean-Fran{\cc}ois Spinella and Mathieu Larivi{\`e}re and Greg Gibson and Anna Andersson and Linda Holmfeldt and Jing Ma and Lei Wei and Jinghui Zhang and Gregor Andelfinger and Downing, {James R} and Mullighan, {Charles G} and Philip Awadalla",
year = "2013",
month = "3",
doi = "10.1101/gr.144188.112",
language = "English",
volume = "23",
pages = "419--30",
journal = "Genome Research",
issn = "1549-5469",
publisher = "Cold Spring Harbor Laboratory Press",
number = "3",