Reaction and strain engineering for improved stereo-selective whole-cell reduction of a bicyclic diketone

Research output: Contribution to journalArticle

Abstract

Reduction of bicyclo[2.2.2]octane-2,6-dione to (1R, 4S, 6S)-6-hydroxy-bicyclo[2.2.2]octane-2-one by whole cells of Saccharomyces cerevisiae was improved using an engineered recombinant strain and process design. The substrate inhibition followed a Han-Levenspiel model showing an effective concentration window between 12 and 22 g/l, in which the activity was kept above 95%. Yeast growth stage, substrate concentration and a stable pH were shown to be important parameters for effective conversion. The over-expression of the reductase gene YDR368w significantly improved diastereoselectivity compared to previously reported results. Using strain TMB4110 expressing YDR368w in batch reduction with pH control, complete conversion of 40 g/l (290 mM) substrate was achieved with 97% diastereomeric excess (de) and >99 enantiomeric excess (ee), allowing isolation of the optically pure ketoalcohol in 84% yield.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Chemical Sciences
  • Industrial Biotechnology
  • Organic Chemistry

Keywords

  • Whole-cell - Bioreduction - Reductase - Yeast - Dicarbonyl - Process optimisation - Toxicity - Substrate inhibition - Diastereoselectivity
Original languageEnglish
Pages (from-to)1111-1118
JournalApplied Microbiology and Biotechnology
Volume77
Issue number5
Publication statusPublished - 2008
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Applied Microbiology (LTH) (011001021), Organic chemistry (S/LTH) (011001240), Center for Chemistry and Chemical Engineering (011001000), Chemical Engineering (011001014)