Reaction medium engineering in enzymatic peptide fragment condensation: Synthesis of eledoisin and LH-RH

Research output: Contribution to journalArticle

Abstract

The influence of different reaction systems on α-chymotrypsin-catalyzed synthesis of eledoisin and LH-RH peptides from (7+4) and (5+5) fragments was investigated. The peptide yield was determined in the following systems: buffered aqueous media, frozen solutions, organic media, and cosolvent mixtures. The experimental set up was tailored to allow the screening of an array of conditions with minimum consumption of peptide fragments (2.1 and 2.5mM). The best yields (22% yield for eledoisin and 68% yield for LH-RH) were obtained in buffered aqueous solutions. It was found that the choice of buffer had a strong influence on the peptide yield; boric-borate and ammonium acetate buffers at pH 9, gave the best results. In buffered aqueous systems, both syntheses were scaled up by using a 10-fold increase in fragment concentration (21 and 25mM). Under these conditions the yields rose to 57% and 80% of eledoisin and LH-RH, respectively. Moreover, during the synthesis of eledoisin and in the presence of boric-borate buffer pH 9, the peptide precipitated from the reaction medium preventing a secondary hydrolysis and facilitating the in situ product purification. Copyright (C) 1998 Elsevier Science Ltd.

Details

Authors
Organisations
External organisations
  • CSIC Centro de Investigación y Desarrollo Pascual Vila (CID)
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biocatalysis and Enzyme Technology

Keywords

  • α-chymotrypsin, Aqueous medium, Biologically active peptides, Cam esters, Eledoisin, Inverse substrate, Kinetically controlled fragment condensation, Luteinising hormone releasing hormone (LH-RH), Trypsin
Original languageEnglish
Pages (from-to)891-901
Number of pages11
JournalBioorganic and Medicinal Chemistry
Volume6
Issue number7
Publication statusPublished - 1998 Jul 1
Publication categoryResearch
Peer-reviewedYes