Real-time Fluorescence Imaging of Capillary Electrophoresis

Research output: Contribution to journalArticle

Abstract

An arrangement for real-time fluorescence imaging of capillary electrophoretic separations is described. A decoated capillary was excited along a substantial part of its length by a dye laser pumped with an XeCl excimer laser tuned to match the fluorophors in the sample. Light emitted from the migrating, fluorescent molecules was detected by an image-intensified, thermoelectrically cooled charge-coupled device camera. The camera signals were processed by a computer and displayed as moving peaks on a screen in real-time, thus allowing the progress of the separation to be monitored continuously. Basic characteristics of the imaging arrangement such as sensitivity (signal-to-noise ratio) and resolution were checked with fluorescein samples. The imaging principle was also illustrated with a separation of a mixture of DNA fragments on a commercially available capillary designated for DNA separations. The benefits of real-time imaging for separation processes, in particular for capillary electrophoresis, are discussed.

Details

Authors
  • S Nilsson
  • Jonas Johansson
  • M Mecklenburg
  • S Birnbaum
  • Sune Svanberg
  • K. G Wahlund
  • K Mosbach
  • A Miyabayashi
  • P. O Larsson
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Atom and Molecular Physics and Optics
Original languageEnglish
Pages (from-to)46-52
JournalJournal of Capillary Electrophoresis
Volume2
Issue number1
Publication statusPublished - 1995
Publication categoryResearch
Peer-reviewedYes