Real-time PCR-assay in the delivery suite for determination of group B streptococcal colonization in a setting with risk-based antibiotic prophylaxis

Research output: Contribution to journalArticle

Standard

Real-time PCR-assay in the delivery suite for determination of group B streptococcal colonization in a setting with risk-based antibiotic prophylaxis. / Hakansson, Stellan; Källén, Karin; Bullarbo, Maria; Holmgren, Per-Ake; Bremme, Katarina; Larsson, Asa; Norman, Margareta; Noren, Hakan; Ortmark-Wrede, Catharina; Pettersson, Karin; Saltvedt, Sissel; Sondell, Birgitta; Tokarska, Magdalena; von Vultee, Anna; Jacobsson, Bo.

In: Journal of Maternal-Fetal & Neonatal Medicine, Vol. 27, No. 4, 2014, p. 328-332.

Research output: Contribution to journalArticle

Harvard

Hakansson, S, Källén, K, Bullarbo, M, Holmgren, P-A, Bremme, K, Larsson, A, Norman, M, Noren, H, Ortmark-Wrede, C, Pettersson, K, Saltvedt, S, Sondell, B, Tokarska, M, von Vultee, A & Jacobsson, B 2014, 'Real-time PCR-assay in the delivery suite for determination of group B streptococcal colonization in a setting with risk-based antibiotic prophylaxis', Journal of Maternal-Fetal & Neonatal Medicine, vol. 27, no. 4, pp. 328-332. https://doi.org/10.3109/14767058.2013.818128

APA

CBE

Hakansson S, Källén K, Bullarbo M, Holmgren P-A, Bremme K, Larsson A, Norman M, Noren H, Ortmark-Wrede C, Pettersson K, Saltvedt S, Sondell B, Tokarska M, von Vultee A, Jacobsson B. 2014. Real-time PCR-assay in the delivery suite for determination of group B streptococcal colonization in a setting with risk-based antibiotic prophylaxis. Journal of Maternal-Fetal & Neonatal Medicine. 27(4):328-332. https://doi.org/10.3109/14767058.2013.818128

MLA

Vancouver

Author

Hakansson, Stellan ; Källén, Karin ; Bullarbo, Maria ; Holmgren, Per-Ake ; Bremme, Katarina ; Larsson, Asa ; Norman, Margareta ; Noren, Hakan ; Ortmark-Wrede, Catharina ; Pettersson, Karin ; Saltvedt, Sissel ; Sondell, Birgitta ; Tokarska, Magdalena ; von Vultee, Anna ; Jacobsson, Bo. / Real-time PCR-assay in the delivery suite for determination of group B streptococcal colonization in a setting with risk-based antibiotic prophylaxis. In: Journal of Maternal-Fetal & Neonatal Medicine. 2014 ; Vol. 27, No. 4. pp. 328-332.

RIS

TY - JOUR

T1 - Real-time PCR-assay in the delivery suite for determination of group B streptococcal colonization in a setting with risk-based antibiotic prophylaxis

AU - Hakansson, Stellan

AU - Källén, Karin

AU - Bullarbo, Maria

AU - Holmgren, Per-Ake

AU - Bremme, Katarina

AU - Larsson, Asa

AU - Norman, Margareta

AU - Noren, Hakan

AU - Ortmark-Wrede, Catharina

AU - Pettersson, Karin

AU - Saltvedt, Sissel

AU - Sondell, Birgitta

AU - Tokarska, Magdalena

AU - von Vultee, Anna

AU - Jacobsson, Bo

PY - 2014

Y1 - 2014

N2 - Objective: Intrapartum antibiotic prophylaxis (IAP) reduces the incidence of neonatal early onset group B streptococcal infections. The present study investigated if an automated PCR-assay, used bedside by the labor ward personnel was manageable and could decrease the use of IAP in a setting with a risk-based IAP strategy. Methods: The study comprises two phases. Phase 1 was a multicenter, randomized, controlled trial. Women with selected risk-factors were allocated either to PCR-IAP (prophylaxis given if positive or indeterminate) or IAP. A vaginal/rectal swab and superficial swabs from the neonate for conventional culture were also obtained. Phase 2 was non-randomized, assessing an improved version of the assay. Results: Phase 1 included 112 women in the PCR-IAP group and 117 in the IAP group. Excluding indeterminate results, the assay showed a sensitivity of 89% and a specificity of 90%. In 44 % of the PCR assays the result was indeterminate. The use of IAP was lower in the PCR group (53 versus 92%). Phase 2 included 94 women. The proportion of indeterminate results was reduced (15%). The GBS colonization rate was 31%. Conclusion: The PCR assay, in the hands of labor ward personnel, can be useful for selection of women to which IAP should be offered.

AB - Objective: Intrapartum antibiotic prophylaxis (IAP) reduces the incidence of neonatal early onset group B streptococcal infections. The present study investigated if an automated PCR-assay, used bedside by the labor ward personnel was manageable and could decrease the use of IAP in a setting with a risk-based IAP strategy. Methods: The study comprises two phases. Phase 1 was a multicenter, randomized, controlled trial. Women with selected risk-factors were allocated either to PCR-IAP (prophylaxis given if positive or indeterminate) or IAP. A vaginal/rectal swab and superficial swabs from the neonate for conventional culture were also obtained. Phase 2 was non-randomized, assessing an improved version of the assay. Results: Phase 1 included 112 women in the PCR-IAP group and 117 in the IAP group. Excluding indeterminate results, the assay showed a sensitivity of 89% and a specificity of 90%. In 44 % of the PCR assays the result was indeterminate. The use of IAP was lower in the PCR group (53 versus 92%). Phase 2 included 94 women. The proportion of indeterminate results was reduced (15%). The GBS colonization rate was 31%. Conclusion: The PCR assay, in the hands of labor ward personnel, can be useful for selection of women to which IAP should be offered.

KW - Antibiotics

KW - delivery

KW - prophylaxis

KW - real-time PCR

KW - S. agalactiae

U2 - 10.3109/14767058.2013.818128

DO - 10.3109/14767058.2013.818128

M3 - Article

VL - 27

SP - 328

EP - 332

JO - Journal of Maternal-Fetal & Neonatal Medicine

T2 - Journal of Maternal-Fetal & Neonatal Medicine

JF - Journal of Maternal-Fetal & Neonatal Medicine

SN - 1476-7058

IS - 4

ER -