Recipient T lymphocytes modulate the severity of antibody-mediated transfusion-related acute lung injury

Research output: Contribution to journalArticle


Transfusion-related acute lung injury (TRALI) is a serious complication of transfusion and has been ranked as one of the leading causes of transfusion-related fatalities. Nonetheless, many details of the immunopathogenesis of TRALI, particularly with respect to recipient factors are unknown. We used a murine model of antibody-mediated TRALI in an attempt to understand the role that recipient lymphocytes might play in TRALI reactions. Intravenous injection of an IgG2a antimurine major histocompatibility complex class I antibody (34-1-2s) into BALB/c mice induced moderate hypothermia and pulmonary granulocyte accumulation but no pulmonary edema nor mortality. In contrast, 34-1-2s injections into mice with severe combined immunodeficiency caused severe hypothermia, severe pulmonary edema, and approximately 40% mortality indicating a critical role for T and B lymphocytes in suppressing TRALI reactions. Adoptive transfer of purified CD8(+) T lymphocytes or CD4(+) T cells but not CD19(+) B cells into the severe combined immunodeficiency mice alleviated the antibody-induced hypothermia, lung damage, and mortality, suggesting that T lymphocytes were responsible for the protective effect. Taken together, these results suggest that recipient T lymphocytes play a significant role in suppressing antibody-mediated TRALI reactions. They identify a potentially new recipient mechanism that controls the severity of TRALI reactions.


  • Yoke Lin Fung
  • Michael Kim
  • Arata Tabuchi
  • Rukhsana Aslam
  • Edwin R Speck
  • Leola Chow
  • Wolfgang M Kuebler
  • John Freedman
  • John W Semple
External organisations
  • University of Queensland
  • Li Ka Shing Knowledge Institute
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Hematology


  • Acute Lung Injury, Animals, Antibodies, Blood Transfusion, Chemokine CXCL2, Histocompatibility Antigens Class I, Hypothermia, Immunoglobulin G, Lung, Lymphocyte Transfusion, Male, Mice, Mice, Inbred BALB C, Mice, SCID, Neutrophils, T-Lymphocytes, Journal Article, Research Support, Non-U.S. Gov't
Original languageEnglish
Pages (from-to)3073-9
Number of pages7
Issue number16
Publication statusPublished - 2010 Oct 21
Publication categoryResearch
Externally publishedYes