Reduced diversity in the early fecal microbiota of infants developing atopic eczema

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title = "Reduced diversity in the early fecal microbiota of infants developing atopic eczema",
abstract = "Background It might be that early intestinal colonization by bacteria in westernized infants fails to give rise to sufficient immune stimulation to support maturation of regulatory immune mechanisms. Objective The purpose of the present study was to characterize the very early infantile microbiota by using a culture-independent approach and to relate the colonization pattern to development of atopic eczema in the first 18 months of life. Methods Fecal samples were collected from 35 infants at 1 week of age. Twenty infants were healthy, and 15 infants were given diagnoses of atopic eczema at the age of 18 months. The fecal microbiota of the infants was compared by means of terminal restriction fragment length polymorphism (T-RFLP) and temporal temperature gradient gel electrophoresis (TTGE) analysis of amplified 16S rRNA genes. Results By means of T-RFLP analysis, the median number of peaks, Shannon-Wiener index, and Simpson index of diversity were significantly less for infants with atopic eczema than for infants remaining healthy in the whole group and for the Swedish infants when AluI was used for digestion. The same was found when TTGE patterns were compared. In addition, TTGE analysis showed significantly less bands and lower diversity indices for the British atopic infants compared with those of the control subjects. Conclusion There is a reduced diversity in the early fecal microbiota of infants with atopic eczema during the first 18 months of life.",
keywords = "Atopic eczema, intestinal microbiota, diversity, terminal restriction fragment length polymorphism, temporal temperature gradient gel electrophoresis",
author = "Mei Wang and Caroline Linninge and Crister Olsson and Ingegerd Adlerberth and Agnes Wold and Strachan, {David P} and Martricardi, {Paolo M} and Nils {\AA}berg and Perkin, {Michael R} and Salvatore Tripodi and Coates, {Anthony R} and Bill Hesselmar and Robert Saalman and G{\"o}ran Molin and Siv Ahrn{\'e}",
note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Applied Nutrition and Food Chemistry (011001300), Surgery Research Unit (013242220), Food Technology (011001017)",
year = "2008",
doi = "10.1016/j.jaci.2007.09.011",
language = "English",
volume = "121",
pages = "129--134",
journal = "Journal of Allergy and Clinical Immunology",
issn = "1097-6825",
publisher = "Elsevier",
number = "1",