Regulation of bone sialoprotein mRNA by steroid hormones.

Research output: Contribution to journalArticle

Abstract

In this report we demonstrate an increase in the steady-state level of bone sialoprotein (BSP) mRNA in rat calvaria and a rat osteosarcoma cell line (ROS 17/2.8) after treatment with the synthetic glucocorticoid, dexamethasone. In contrast, 1.25-dihydroxyvitamin D3 reduced the amount of BSP mRNA in calvaria and inhibited the dexamethasone induction in ROS 17/2.8 cells. The increase in BSP mRNA is most likely due to an increase in the transcriptional rate. The stability of mRNA was unchanged after dexamethasone treatment with a half-life of approximately 5 h. Nuclear transcription experiments with nuclei isolated from ROS 17/2.8 cells showed an increased BSP mRNA synthesis in cells treated with dexamethasone.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Otorhinolaryngology

Keywords

  • Messenger: genetics, Messenger: drug effects, RNA, Genes: drug effects, Gene Expression Regulation: drug effects, Dexamethasone: pharmacology, Calcitriol: pharmacology, Bone and Bones: drug effects, Bone and Bones: metabolism, Sialoglycoproteins: genetics, Transcription, Genetic: drug effects
Original languageEnglish
Pages (from-to)3183-3186
JournalJournal of Cell Biology
Volume109
Issue number6 Pt 1
Publication statusPublished - 1989
Publication categoryResearch
Peer-reviewedYes