Relationship between condensed tannin structures and their ability to precipitate feed proteins in the rumen

Research output: Contribution to journalArticle

Abstract

BACKGROUND Tannins can bind to and precipitate protein by forming insoluble complexes resistant to fermentation and with a positive effect on protein utilisation by ruminants. Three protein types, Rubisco, rapeseed protein and bovine serum albumin (a single high-molecular weight protein), were used to test the effects of increasing concentrations of structurally different condensed tannins on protein solubility/precipitation. RESULTS Protein type (PT) influenced solubility after addition of condensed tannins (P < 0.001) in the order: Rubisco < rapeseed < BSA (P < 0.05). The type of condensed tannin (CT) affected protein solubility (P = 0.001) with a CT x PT interaction (P = 0.001). Mean degree of polymerisation, proportions of cis- versus trans-flavanol subunits or prodelphinidins versus procyanidins among CTs could not explain precipitation capacities. Increasing tannin concentration decreased protein solubility (P < 0.001) with a PT x CT concentration interaction. The proportion of low-molecular weight rapeseed proteins remaining in solution increased with CT concentration but not with Rubisco. CONCLUSIONS Results of this study suggest that PT and CT type are both of importance for protein precipitation but that the CT structures investigated did not allow identification of parameters that contribute most to precipitation. It is possible that the three-dimensional structures of tannins and proteins may be more important factors in tannin-protein interactions. (c) 2013 Society of Chemical Industry

Details

Authors
  • Martin M. Lorenz
  • Loy Alkhafadji
  • Elisabetta Stringano
  • Staffan Nilsson
  • Irene Mueller-Harvey
  • Peter Uden
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Agricultural Science, Forestry and Fisheries

Keywords

  • Lab on Chip, Rubisco, flavanol composition, bovine serum albumin, condensed tannins, rape seed
Original languageEnglish
Pages (from-to)963-968
JournalJournal of the Science of Food and Agriculture
Volume94
Issue number5
Publication statusPublished - 2014
Publication categoryResearch
Peer-reviewedYes