Repetitive organic dust exposure in vitro impairs macrophage differentiation and function

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Repetitive organic dust exposure in vitro impairs macrophage differentiation and function. / Poole, Jill A.; Alexis, Neil E.; Parks, Conrad; MacInnes, Amy K.; Gentry-Nielsen, Martha J.; Fey, Paul D.; Larsson, Lennart; Allen-Gipson, Diane; Von Essen, Susanna G.; Romberger, Debra J.

In: Journal of Allergy and Clinical Immunology, Vol. 122, No. 2, 2008, p. 375-382.

Research output: Contribution to journalArticle

Harvard

Poole, JA, Alexis, NE, Parks, C, MacInnes, AK, Gentry-Nielsen, MJ, Fey, PD, Larsson, L, Allen-Gipson, D, Von Essen, SG & Romberger, DJ 2008, 'Repetitive organic dust exposure in vitro impairs macrophage differentiation and function', Journal of Allergy and Clinical Immunology, vol. 122, no. 2, pp. 375-382. https://doi.org/10.1016/j.jaci.2008.05.023

APA

Poole, J. A., Alexis, N. E., Parks, C., MacInnes, A. K., Gentry-Nielsen, M. J., Fey, P. D., ... Romberger, D. J. (2008). Repetitive organic dust exposure in vitro impairs macrophage differentiation and function. Journal of Allergy and Clinical Immunology, 122(2), 375-382. https://doi.org/10.1016/j.jaci.2008.05.023

CBE

Poole JA, Alexis NE, Parks C, MacInnes AK, Gentry-Nielsen MJ, Fey PD, Larsson L, Allen-Gipson D, Von Essen SG, Romberger DJ. 2008. Repetitive organic dust exposure in vitro impairs macrophage differentiation and function. Journal of Allergy and Clinical Immunology. 122(2):375-382. https://doi.org/10.1016/j.jaci.2008.05.023

MLA

Vancouver

Author

Poole, Jill A. ; Alexis, Neil E. ; Parks, Conrad ; MacInnes, Amy K. ; Gentry-Nielsen, Martha J. ; Fey, Paul D. ; Larsson, Lennart ; Allen-Gipson, Diane ; Von Essen, Susanna G. ; Romberger, Debra J. / Repetitive organic dust exposure in vitro impairs macrophage differentiation and function. In: Journal of Allergy and Clinical Immunology. 2008 ; Vol. 122, No. 2. pp. 375-382.

RIS

TY - JOUR

T1 - Repetitive organic dust exposure in vitro impairs macrophage differentiation and function

AU - Poole, Jill A.

AU - Alexis, Neil E.

AU - Parks, Conrad

AU - MacInnes, Amy K.

AU - Gentry-Nielsen, Martha J.

AU - Fey, Paul D.

AU - Larsson, Lennart

AU - Allen-Gipson, Diane

AU - Von Essen, Susanna G.

AU - Romberger, Debra J.

PY - 2008

Y1 - 2008

N2 - Organic dust exposure in the agricultural industry results in significant airway disease and lung function decrease. Mononuclear phagocytes are key cells that mediate the inflammatory and innate immune response after dust exposure. Objective: We sought to investigate the effect of organic dust extract (ODE) from modern swine operations on monocyte-derived macrophage (MDM) phenotype and function. Methods: Peripheral blood monocytes were obtained by means of elutriation methodology (> 99% CD14(+)) and differentiated into macrophages in the presence of GM-CSF (1 week) with and without ODE (0.1 %). At 1 week, cells were analyzed by means of flow cytometry for cell-surface marker expression (HLA-DR, CD80, CD86, Toll-like receptor 2, Toll-like receptor 4, mCD14, and CD16), phagocytosis (IgG-opsonized zymosan particles), and intracellular killing of Streptococcus pneumoniae. At 1 week, MDMs were rechallenged with high-dose ODE (1%), LPS, and peptidoglycan (PGN), and cytokine levels TNF-alpha, IL-6, IL-10, and CXCL8/IL-8) were measured. Comparisons were made to MDMs conditioned with heat-inactivated dust, endotoxin-depleted dust, LPS, and PGN to elucidate ODE-associated factors. Results: Expression of HLA-DR, CD80, and CD86; phagocytosis; and intracellular bacterial killing were significantly decreased with ODE-challenged versus control MDMs. Responses were retained after marked depletion of endotoxin. PGN, LPS, and PGN plus LPS significantly reduced MDM surface marker expression and, except for LPS alone, also reduced phagocytosis. ODE-challenged MDMs had significantly diminished cytokine responses (TNF-alpha, IL-6, and IL-10) after repeat challenge with high-dose ODE. Cross-tolerant cytokine responses were also observed. Conclusion: Repetitive organic dust exposure significantly decreases markers of antigen presentation and host defense function in MDMs. Bacterial cell components appear to be driving these impaired responses.

AB - Organic dust exposure in the agricultural industry results in significant airway disease and lung function decrease. Mononuclear phagocytes are key cells that mediate the inflammatory and innate immune response after dust exposure. Objective: We sought to investigate the effect of organic dust extract (ODE) from modern swine operations on monocyte-derived macrophage (MDM) phenotype and function. Methods: Peripheral blood monocytes were obtained by means of elutriation methodology (> 99% CD14(+)) and differentiated into macrophages in the presence of GM-CSF (1 week) with and without ODE (0.1 %). At 1 week, cells were analyzed by means of flow cytometry for cell-surface marker expression (HLA-DR, CD80, CD86, Toll-like receptor 2, Toll-like receptor 4, mCD14, and CD16), phagocytosis (IgG-opsonized zymosan particles), and intracellular killing of Streptococcus pneumoniae. At 1 week, MDMs were rechallenged with high-dose ODE (1%), LPS, and peptidoglycan (PGN), and cytokine levels TNF-alpha, IL-6, IL-10, and CXCL8/IL-8) were measured. Comparisons were made to MDMs conditioned with heat-inactivated dust, endotoxin-depleted dust, LPS, and PGN to elucidate ODE-associated factors. Results: Expression of HLA-DR, CD80, and CD86; phagocytosis; and intracellular bacterial killing were significantly decreased with ODE-challenged versus control MDMs. Responses were retained after marked depletion of endotoxin. PGN, LPS, and PGN plus LPS significantly reduced MDM surface marker expression and, except for LPS alone, also reduced phagocytosis. ODE-challenged MDMs had significantly diminished cytokine responses (TNF-alpha, IL-6, and IL-10) after repeat challenge with high-dose ODE. Cross-tolerant cytokine responses were also observed. Conclusion: Repetitive organic dust exposure significantly decreases markers of antigen presentation and host defense function in MDMs. Bacterial cell components appear to be driving these impaired responses.

KW - cell surface molecules

KW - killing

KW - intracellular

KW - phagocytosis

KW - organic dust

KW - monocyte

KW - macrophage

KW - peptidoglycan

KW - LPS

KW - inflammation

KW - cytokines

U2 - 10.1016/j.jaci.2008.05.023

DO - 10.1016/j.jaci.2008.05.023

M3 - Article

VL - 122

SP - 375

EP - 382

JO - Journal of Allergy and Clinical Immunology

T2 - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 1097-6825

IS - 2

ER -