Respiration of [C-14] alanine by the ectomycorrhizal fungus Paxillus involutus
Research output: Contribution to journal › Article
The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [C-14]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [C-14]alanine was further actively metabolised to glutamate, glutamine and aspartate. Exposure to aminooxyacetate completely suppressed (CO2)-C-14 evolution and greatly reduced the flow of carbon from [C-14]alanine to tricarboxylic acid cycle intermediates and amino acids, suggesting that alanine aminotransferase plays a pivotal role in alanine metabolism in Paxillus involutus.
|Research areas and keywords||
Subject classification (UKÄ) – MANDATORY
|Journal||FEMS Microbiology Letters|
|Publication status||Published - 1994|