Respiration of [C-14] alanine by the ectomycorrhizal fungus Paxillus involutus

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Abstract

The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [C-14]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [C-14]alanine was further actively metabolised to glutamate, glutamine and aspartate. Exposure to aminooxyacetate completely suppressed (CO2)-C-14 evolution and greatly reduced the flow of carbon from [C-14]alanine to tricarboxylic acid cycle intermediates and amino acids, suggesting that alanine aminotransferase plays a pivotal role in alanine metabolism in Paxillus involutus.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biological Sciences

Keywords

  • Amino acid respiration, Aminooxyacetate, [14C]alanine, Ectomycorrhizal fungus, Paxillus involutus, TCA cycle
Original languageEnglish
Pages (from-to)87-91
JournalFEMS Microbiology Letters
Volume121
Issue number1
Publication statusPublished - 1994
Publication categoryResearch
Peer-reviewedYes