Results of the first World Health Organization international collaborative study of detection of Human Papillomavirus DNA.

Research output: Contribution to journalArticle


Twenty-nine laboratories in 12 countries participated in a study to assess the performance of various human
papillomavirus (HPV) detection assays through the use of a recombinant HPV DNA standard reagent panel.
The panel was designed by a group of HPV experts, and samples were prepared and distributed by the World
Health Organization International Laboratory for Standards and Biologicals in The Netherlands. Each panel
consisted of 24 coded samples including a dilution series for HPV types 16 and 18, alone or in combination with
five other high-risk (HR) HPV types including HPV types 31, 33, 35, 45, and 52, the low-risk HPV type 6, and
a negative control. Qualitative assays were generally consistent across laboratories, and most invalid results
reflected a lack of HPV test sensitivity. The combined data sets had a proficiency for HPV 16 of 62.5% (15/24)
and for HPV 18 of 73.9% (17/23). HPV 31 was the least accurately detected by participating laboratories.
Approximately half of participating laboratories failed to detect high concentrations of HPV 31 and, to a lesser
extent, to detect HPV types 35, 52, and 6. The panel sample materials offer a source of renewable and
reproducible material that could be used in the future development of international standard reagents for
calibration of HPV DNA assays and kits.


  • W G V Quint
  • S Pagliusi
  • N Lelie
  • E M de Villiers
  • C M Wheeler
  • Joakim Dillner
  • World Health Organization Human Papillomavirus DNA international collaborative study group. Human Papillomavirus DNA international collaborative study group.
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Microbiology in the medical area
Original languageEnglish
Pages (from-to)571-579
JournalJournal of Clinical Microbiology
Issue number2
Publication statusPublished - 2006
Publication categoryResearch