Results of the first World Health Organization international collaborative study of detection of Human Papillomavirus DNA.

Research output: Contribution to journalArticle

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Results of the first World Health Organization international collaborative study of detection of Human Papillomavirus DNA. / Quint, W G V; Pagliusi, S; Lelie, N; de Villiers, E M; Wheeler, C M; Dillner, Joakim; Human Papillomavirus DNA international collaborative study group., World Health Organization Human Papillomavirus DNA international collaborative study group.

In: Journal of Clinical Microbiology, Vol. 44, No. 2, 2006, p. 571-579.

Research output: Contribution to journalArticle

Harvard

Quint, WGV, Pagliusi, S, Lelie, N, de Villiers, EM, Wheeler, CM, Dillner, J & Human Papillomavirus DNA international collaborative study group., WHOHPDNAICSG 2006, 'Results of the first World Health Organization international collaborative study of detection of Human Papillomavirus DNA.', Journal of Clinical Microbiology, vol. 44, no. 2, pp. 571-579. https://doi.org/10.1128/JCM.44.2.571–579.2006

APA

Quint, W. G. V., Pagliusi, S., Lelie, N., de Villiers, E. M., Wheeler, C. M., Dillner, J., & Human Papillomavirus DNA international collaborative study group., W. H. O. H. P. DNA. I. C. S. G. (2006). Results of the first World Health Organization international collaborative study of detection of Human Papillomavirus DNA. Journal of Clinical Microbiology, 44(2), 571-579. https://doi.org/10.1128/JCM.44.2.571–579.2006

CBE

Quint WGV, Pagliusi S, Lelie N, de Villiers EM, Wheeler CM, Dillner J, Human Papillomavirus DNA international collaborative study group. WHOHPDNAICSG. 2006. Results of the first World Health Organization international collaborative study of detection of Human Papillomavirus DNA. Journal of Clinical Microbiology. 44(2):571-579. https://doi.org/10.1128/JCM.44.2.571–579.2006

MLA

Vancouver

Author

Quint, W G V ; Pagliusi, S ; Lelie, N ; de Villiers, E M ; Wheeler, C M ; Dillner, Joakim ; Human Papillomavirus DNA international collaborative study group., World Health Organization Human Papillomavirus DNA international collaborative study group. / Results of the first World Health Organization international collaborative study of detection of Human Papillomavirus DNA. In: Journal of Clinical Microbiology. 2006 ; Vol. 44, No. 2. pp. 571-579.

RIS

TY - JOUR

T1 - Results of the first World Health Organization international collaborative study of detection of Human Papillomavirus DNA.

AU - Quint, W G V

AU - Pagliusi, S

AU - Lelie, N

AU - de Villiers, E M

AU - Wheeler, C M

AU - Dillner, Joakim

AU - Human Papillomavirus DNA international collaborative study group., World Health Organization Human Papillomavirus DNA international collaborative study group.

PY - 2006

Y1 - 2006

N2 - Twenty-nine laboratories in 12 countries participated in a study to assess the performance of various human papillomavirus (HPV) detection assays through the use of a recombinant HPV DNA standard reagent panel. The panel was designed by a group of HPV experts, and samples were prepared and distributed by the World Health Organization International Laboratory for Standards and Biologicals in The Netherlands. Each panel consisted of 24 coded samples including a dilution series for HPV types 16 and 18, alone or in combination with five other high-risk (HR) HPV types including HPV types 31, 33, 35, 45, and 52, the low-risk HPV type 6, and a negative control. Qualitative assays were generally consistent across laboratories, and most invalid results reflected a lack of HPV test sensitivity. The combined data sets had a proficiency for HPV 16 of 62.5% (15/24) and for HPV 18 of 73.9% (17/23). HPV 31 was the least accurately detected by participating laboratories. Approximately half of participating laboratories failed to detect high concentrations of HPV 31 and, to a lesser extent, to detect HPV types 35, 52, and 6. The panel sample materials offer a source of renewable and reproducible material that could be used in the future development of international standard reagents for calibration of HPV DNA assays and kits.

AB - Twenty-nine laboratories in 12 countries participated in a study to assess the performance of various human papillomavirus (HPV) detection assays through the use of a recombinant HPV DNA standard reagent panel. The panel was designed by a group of HPV experts, and samples were prepared and distributed by the World Health Organization International Laboratory for Standards and Biologicals in The Netherlands. Each panel consisted of 24 coded samples including a dilution series for HPV types 16 and 18, alone or in combination with five other high-risk (HR) HPV types including HPV types 31, 33, 35, 45, and 52, the low-risk HPV type 6, and a negative control. Qualitative assays were generally consistent across laboratories, and most invalid results reflected a lack of HPV test sensitivity. The combined data sets had a proficiency for HPV 16 of 62.5% (15/24) and for HPV 18 of 73.9% (17/23). HPV 31 was the least accurately detected by participating laboratories. Approximately half of participating laboratories failed to detect high concentrations of HPV 31 and, to a lesser extent, to detect HPV types 35, 52, and 6. The panel sample materials offer a source of renewable and reproducible material that could be used in the future development of international standard reagents for calibration of HPV DNA assays and kits.

U2 - 10.1128/JCM.44.2.571–579.2006

DO - 10.1128/JCM.44.2.571–579.2006

M3 - Article

VL - 44

SP - 571

EP - 579

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 1098-660X

IS - 2

ER -