Review of life science applications using submicron O-PTIR and simultaneous Raman microscopy: a new paradigm in vibrational spectroscopy

Research output: Contribution to journalReview article


The recent advent of Optical Photothermal IR (O-PTIR), has enabled for the first time, submicron infrared microscopy in far-field reflection mode with the combination of Raman for simultaneous, correlative IR+Raman microscopy. These unique and exciting synergistic capabilities are now spawning interest in life science application. A broad range of life science applications, otherwise impossible with traditional FTIR/QCL microscopy, will be presented, ranging from live cell imaging in water, to ultra-high resolution images of breast tissue calcifications, amyloid aggregates in neurons (neurites and dendritic spines), individual collagen fibrils with polarized IR and individual isotopically labelled bacterial cells and more.


  • Mustafa Kanziz
  • Alice Spadea
  • Jayakrupakar Nallala
  • Cassio Lima
  • Howbeer Muhamadali
  • Joanna Denbigh
  • Jayne Lawrence
  • Gorkem Bakir
  • Peter Gardner
  • Nick Stone
  • Roy Goodacre
  • Kathleen Gough
  • Oxana Klementieva
External organisations
  • Photothermal Spectroscopy Corporation
  • University of Manchester
  • University of Exeter
  • University of Liverpool
  • University of Salford
  • University of Manitoba
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Atom and Molecular Physics and Optics
Original languageEnglish
Article number1165617
JournalAdvanced Chemical Microscopy for Life Science and Translational Medicine
Publication statusPublished - 2021 Mar 5
Publication categoryResearch
Duration: 2021 Mar 62021 Apr 12

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