Review of life science applications using submicron O-PTIR and simultaneous Raman microscopy: a new paradigm in vibrational spectroscopy

Research output: Contribution to journalReview article

Abstract

The recent advent of Optical Photothermal IR (O-PTIR), has enabled for the first time, submicron infrared microscopy in far-field reflection mode with the combination of Raman for simultaneous, correlative IR+Raman microscopy. These unique and exciting synergistic capabilities are now spawning interest in life science application. A broad range of life science applications, otherwise impossible with traditional FTIR/QCL microscopy, will be presented, ranging from live cell imaging in water, to ultra-high resolution images of breast tissue calcifications, amyloid aggregates in neurons (neurites and dendritic spines), individual collagen fibrils with polarized IR and individual isotopically labelled bacterial cells and more.

Details

Authors
  • Mustafa Kanziz
  • Alice Spadea
  • Jayakrupakar Nallala
  • Cassio Lima
  • Howbeer Muhamadali
  • Joanna Denbigh
  • Jayne Lawrence
  • Gorkem Bakir
  • Peter Gardner
  • Nick Stone
  • Roy Goodacre
  • Kathleen Gough
  • Oxana Klementieva
Organisations
External organisations
  • Photothermal Spectroscopy Corporation
  • University of Manchester
  • University of Exeter
  • University of Liverpool
  • University of Salford
  • University of Manitoba
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Atom and Molecular Physics and Optics
Original languageEnglish
Article number1165617
JournalAdvanced Chemical Microscopy for Life Science and Translational Medicine
Volume11656
Publication statusPublished - 2021 Mar 5
Publication categoryResearch
Peer-reviewedYes
EventSPIE BIOS -
Duration: 2021 Mar 62021 Apr 12
https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11656.toc

Bibliographic note

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