Revised transmembrane orientation of the NADH:quinone oxidoreductase subunit NuoA.

Research output: Contribution to journalArticle

Abstract

NuoA is a small membrane spanning subunit of respiratory chain NADH:quinone oxidoreductase (complex I). Unlike the other complex I core protein subunits, the NuoA protein has no known homologue in other enzyme systems. The transmembrane orientation of NuoA cannot be unambiguously predicted, due to the small size of the polypeptide and the varying distribution of charged amino acid residues in NuoA from different organisms. Novel analyses of NuoA from Escherichia coli complex I expressed as fusion proteins to cytochrome c and to alkaline phosphatase demonstrated that the c-terminal end of the polypeptide is localized in the bacterial cytoplasm, in contrast to what was previously reported for the homologous NQO7 subunit from Paracoccus denitrificans complex I.

Details

Authors
  • Egle Virzintiene
  • Maria Trane
  • Cecilia Hägerhäll
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biological Sciences
Original languageEnglish
Pages (from-to)3277-3283
JournalFEBS Letters
Volume585
Publication statusPublished - 2011
Publication categoryResearch
Peer-reviewedYes