Robotic implementation of a microchip-based protein clean-up and enrichment system for MALDI-TOF MS readout

Research output: Contribution to journalArticle

Abstract

A capillary filling microfluidic proteomic sample processing system has been realized in an automated set-up. Chip-integrated solid-phase extraction is performed in a 96-array format followed by sequential capillary action elution into a piezoelectric microdispenser and subsequent transfer to MALDI targets. Samples are eluted and deposited in volumes of 200–300 nl. The robotic system offers calibration to user-defined microextraction arrays and MALDI-target formats at micrometre resolution. Built-in force feedback control ensures a precise and robust microchip docking/handling in three dimensions. An efficient automated washing protocol eliminates analyte carry-over. System throughput ranges typically from 50–100 samples h-1.

Details

Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Analytical Chemistry
  • Medical Engineering

Keywords

  • piezodispensing, capillary force, microfluidics, MALDI, mass spectrometry, microchip, robotic, MALDI-TOF MS, automation, protein analysis, proteomics, solid phase extraction
Original languageEnglish
Pages (from-to)3147-3153
JournalMeasurement Science & Technology
Volume17
Issue number12
Publication statusPublished - 2006
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biomedical Engineering (011200011), Robotics (ceased) (LUR000026), Analytical Chemistry (S/LTH) (011001004), Departments at LTH (011200000)