Role of thiol groups in insulin release: studies with poorly permeating disulphides

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Role of thiol groups in insulin release : studies with poorly permeating disulphides. / Hellman, B.; Idahl, L. A.; Lernmark, A.; Sehlin, J.; Täljedal, I. B.

In: Molecular Pharmacology, Vol. 9, No. 6, 01.12.1973, p. 792-801.

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Hellman, B, Idahl, LA, Lernmark, A, Sehlin, J & Täljedal, IB 1973, 'Role of thiol groups in insulin release: studies with poorly permeating disulphides', Molecular Pharmacology, vol. 9, no. 6, pp. 792-801.

APA

Hellman, B., Idahl, L. A., Lernmark, A., Sehlin, J., & Täljedal, I. B. (1973). Role of thiol groups in insulin release: studies with poorly permeating disulphides. Molecular Pharmacology, 9(6), 792-801.

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Hellman, B. ; Idahl, L. A. ; Lernmark, A. ; Sehlin, J. ; Täljedal, I. B. / Role of thiol groups in insulin release : studies with poorly permeating disulphides. In: Molecular Pharmacology. 1973 ; Vol. 9, No. 6. pp. 792-801.

RIS

TY - JOUR

T1 - Role of thiol groups in insulin release

T2 - studies with poorly permeating disulphides

AU - Hellman, B.

AU - Idahl, L. A.

AU - Lernmark, A.

AU - Sehlin, J.

AU - Täljedal, I. B.

PY - 1973/12/1

Y1 - 1973/12/1

N2 - At a concentration of 1.0 mM, 6,6' dithiodinicotinic acid and 5,5' dithiobis (2 nitrobenzoic acid) stimulated insulin release from microdissected pancreatic islets of hereditary obese (ob/ob) mice. Microperifusion experiments showed that the secretory responses occurred promptly upon exposure to the sulfhydryl reagents. Perifusion with 6,6' dithiodinicotinic acid induced a sustained enhancement of insulin release without any signs of multiphasic secretion. This reagent induced a similar release pattern at both 3 and 17 mM glucose, although the high glucose concentration appeared to potentiate the effect of 6,6' dithiodinicotinic acid. The dynamics of insulin release in response to 5,5' dithiobis (2 nitrobenzoic acid) depended markedly, however, on the accompanying glucose concentration. At 0 and 3 mM glucose an initial peak of release was followed by a steady decline towards the basal release rate, whereas at 17 mM glucose 5,5' dithiobis (2 nitrobenzoic acid) produced a sustained enhancement of secretion. The omission of calcium significantly inhibited insulin release in response of either of the two disulfides, although clear cut stimulation was still obtained. In marked contrast, 17 glucose clucose did not elicit even an initial peak of insulin release when calcium was omitted from the perifusion medium. 6,6' Dithiodinicotinic acid (0.01-1.0 mM) had no effect on the oxidation of (U 14C)D glucose. Significant inhibition of glucose oxidation was obtained with 0.1 and 1.0 mM 5,5' dithiobis (2 nitrobenzoic acid), whereas at a concentration of 0.01 mM this reagent stimulated oxidation. Since the disulfides do not readily penetrate cell membranes, the results are consistent with the hypothesis that insulin release is regulated by relatively superficial thiol groups in the β cell plasma membrane. However, further studies are necessary to exclude the possibility that the observed effects were due to small amounts of disulphide entering the β cells.

AB - At a concentration of 1.0 mM, 6,6' dithiodinicotinic acid and 5,5' dithiobis (2 nitrobenzoic acid) stimulated insulin release from microdissected pancreatic islets of hereditary obese (ob/ob) mice. Microperifusion experiments showed that the secretory responses occurred promptly upon exposure to the sulfhydryl reagents. Perifusion with 6,6' dithiodinicotinic acid induced a sustained enhancement of insulin release without any signs of multiphasic secretion. This reagent induced a similar release pattern at both 3 and 17 mM glucose, although the high glucose concentration appeared to potentiate the effect of 6,6' dithiodinicotinic acid. The dynamics of insulin release in response to 5,5' dithiobis (2 nitrobenzoic acid) depended markedly, however, on the accompanying glucose concentration. At 0 and 3 mM glucose an initial peak of release was followed by a steady decline towards the basal release rate, whereas at 17 mM glucose 5,5' dithiobis (2 nitrobenzoic acid) produced a sustained enhancement of secretion. The omission of calcium significantly inhibited insulin release in response of either of the two disulfides, although clear cut stimulation was still obtained. In marked contrast, 17 glucose clucose did not elicit even an initial peak of insulin release when calcium was omitted from the perifusion medium. 6,6' Dithiodinicotinic acid (0.01-1.0 mM) had no effect on the oxidation of (U 14C)D glucose. Significant inhibition of glucose oxidation was obtained with 0.1 and 1.0 mM 5,5' dithiobis (2 nitrobenzoic acid), whereas at a concentration of 0.01 mM this reagent stimulated oxidation. Since the disulfides do not readily penetrate cell membranes, the results are consistent with the hypothesis that insulin release is regulated by relatively superficial thiol groups in the β cell plasma membrane. However, further studies are necessary to exclude the possibility that the observed effects were due to small amounts of disulphide entering the β cells.

UR - http://www.scopus.com/inward/record.url?scp=0015755387&partnerID=8YFLogxK

M3 - Article

C2 - 4587007

AN - SCOPUS:0015755387

VL - 9

SP - 792

EP - 801

JO - Molecular Pharmacology

JF - Molecular Pharmacology

SN - 1521-0111

IS - 6

ER -