Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa

Research output: Contribution to journalArticle

Abstract

Secretory leukocyte protease inhibitor (SLPI) is a well-known protease inhibitor. Its function is thought to be protease/protease-inhibitor balance. Free proteolytic activity, mainly pancreatic elastase, anionic trypsin and granulocytic elastase, has been demonstrated in faecal extracts from patients with ulcerative colitis. We wanted to verify that SLPI is actually secreted from normal human colonic mucosa. Also, we wanted to ascertain whether studies of SLPI secretion based on punch biopsies were dependent on biopsy area or on biopsy circumference. Normal colonic mucosa was sampled during surgery for colonic cancer. A total of 36 samples from four patients were used. Mucosa preparation was carried out using a punch biopsy technique, and samples of 3, 4 and 6 mm diameter were used. All media contained SLPI at varying concentrations. When expressed in terms of the sample area, the secretion per millimetre-squared seemed to decrease with increasing area. When calculated as secretion per circumference, secretion seemed to be constant. In conclusion, SLPI was secreted from normal human colonic mucosa. The SLPI secretion seemed dependent on the circumference of the biopsy rather than on the area of the biopsy.

Details

Authors
Organisations
External organisations
  • Skåne University Hospital
Research areas and keywords

Keywords

  • Anti-leukoprotease, Human cell culture, Punch biopsy, Trauma
Original languageEnglish
Pages (from-to)269-272
JournalMediators of Inflammation
Volume10
Issue number5
Publication statusPublished - 2001
Publication categoryResearch
Peer-reviewedYes