Selective infection of E. coli as a function of a specific molecular interaction.

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Selective infection of E. coli as a function of a specific molecular interaction. / Nilsson, Nina; Karlsson, Fredrik; Rakonjac, Jasna; Borrebaeck, Carl.

In: Journal of Molecular Recognition, Vol. 15, No. 1, 2002, p. 27-32.

Research output: Contribution to journalArticle

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Nilsson, Nina ; Karlsson, Fredrik ; Rakonjac, Jasna ; Borrebaeck, Carl. / Selective infection of E. coli as a function of a specific molecular interaction. In: Journal of Molecular Recognition. 2002 ; Vol. 15, No. 1. pp. 27-32.

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TY - JOUR

T1 - Selective infection of E. coli as a function of a specific molecular interaction.

AU - Nilsson, Nina

AU - Karlsson, Fredrik

AU - Rakonjac, Jasna

AU - Borrebaeck, Carl

PY - 2002

Y1 - 2002

N2 - Selective infection of phage is when the bacterial infection depends on the specific molecular interaction between an antigen and a phage-displayed protein sequence such as an antibody. Engineering of the normal infection into pathways, directed by a specific protein--protein interaction, has raised several mechanistic questions. Here, we address the type of display and the affinity between the interacting pairs. The deleted phage R408d3 was used for the first time in selective infection and was shown to exhibit a superior performance compared to the VCSM13 phage. Furthermore, the affinity between the interacting pairs also affected the selective infection process and a correlation between affinity and infection efficiency was detected, thus implying that selective infection is the method of choice for selection of rare high-affinity interactions in molecular libraries.

AB - Selective infection of phage is when the bacterial infection depends on the specific molecular interaction between an antigen and a phage-displayed protein sequence such as an antibody. Engineering of the normal infection into pathways, directed by a specific protein--protein interaction, has raised several mechanistic questions. Here, we address the type of display and the affinity between the interacting pairs. The deleted phage R408d3 was used for the first time in selective infection and was shown to exhibit a superior performance compared to the VCSM13 phage. Furthermore, the affinity between the interacting pairs also affected the selective infection process and a correlation between affinity and infection efficiency was detected, thus implying that selective infection is the method of choice for selection of rare high-affinity interactions in molecular libraries.

KW - Protein Engineering

KW - Peptide Library

KW - Escherichia coli Infections/microbiology

KW - Bacteriophages/metabolism/physiology

KW - Escherichia coli/metabolism/physiology

KW - Support

KW - Non-U.S. Gov't

KW - U.S. Gov't

KW - Non-P.H.S.

U2 - 10.1002/jmr.557

DO - 10.1002/jmr.557

M3 - Article

VL - 15

SP - 27

EP - 32

JO - Journal of Molecular Recognition

T2 - Journal of Molecular Recognition

JF - Journal of Molecular Recognition

SN - 1099-1352

IS - 1

ER -