Selective nucleotide-release from dense-core granules in insulin-secreting cells.
Research output: Contribution to journal › Article
Secretory granules of insulin-secreting cells are used to store and release peptide hormones as well as low-molecular-weight compounds such as nucleotides. Here we have compared the rate of exocytosis with the time courses of nucleotide and peptide release by a combination of capacitance measurements, electrophysiological detection of ATP release and single-granule imaging. We demonstrate that the release of nucleotides and peptides is delayed by similar to 0.1 and similar to 2 seconds with respect to membrane fusion, respectively. We further show that in up to 70% of the cases exocytosis does not result in significant release of the peptide cargo, likely because of a mechanism that leads to premature closure of the fusion pore. Release of nucleotides and protons occurred regardless of whether peptides were secreted or not. These observations suggest that insulin-secreting cells are able to use the same secretory vesicles to release small molecules either alone or together with the peptide hormone.
|Research areas and keywords||
Subject classification (UKÄ) – MANDATORY
|Journal||Journal of Cell Science|
|Issue number||Pt 18|
|Publication status||Published - 2005|
Related research output
2008, Department of Clinical Sciences, Lund University. 101 p.
Research output: Thesis › Doctoral Thesis (compilation)