Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes: VAMP2 and NPY are new minor autoantigens

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Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes: VAMP2 and NPY are new minor autoantigens. / Hirai, Hiroki; Miura, Junnosuke; Hu, Yafang; Larsson, Helena; Larsson, Karin; Lernmark, Åke; Ivarsson, Sten; Wu, Tianxia; Kingman, Albert; Tzioufas, Athanasios G; Notkins, Abner L.

In: Clinical Immunology, Vol. 127, No. 3, 2008, p. 366-374.

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Hirai, Hiroki ; Miura, Junnosuke ; Hu, Yafang ; Larsson, Helena ; Larsson, Karin ; Lernmark, Åke ; Ivarsson, Sten ; Wu, Tianxia ; Kingman, Albert ; Tzioufas, Athanasios G ; Notkins, Abner L. / Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes: VAMP2 and NPY are new minor autoantigens. In: Clinical Immunology. 2008 ; Vol. 127, No. 3. pp. 366-374.

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TY - JOUR

T1 - Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes: VAMP2 and NPY are new minor autoantigens

AU - Hirai, Hiroki

AU - Miura, Junnosuke

AU - Hu, Yafang

AU - Larsson, Helena

AU - Larsson, Karin

AU - Lernmark, Åke

AU - Ivarsson, Sten

AU - Wu, Tianxia

AU - Kingman, Albert

AU - Tzioufas, Athanasios G

AU - Notkins, Abner L

PY - 2008

Y1 - 2008

N2 - The four major autoantigens (IA-2, IA-2 beta, GAD65 and insulin) of type 1 diabetes are all associated with dense core or synaptic vesicles. This raised the possibility that other secretory vesicle-associated proteins might be targets of the autoimmune response in type 1 diabetes. To test this hypothesis 56 proteins, two-thirds of which are associated with secretory vesicles, were prepared by in vitro transcription/translation and screened for autoantibodies by liquid phase radioimmunoprecipitation. Two secretory vesicle-associated proteins, VAMP2 and NPY, were identified as new minor autoantigens with 21% and 9%, respectively, of 200 type 1 diabetes sera reacting positively. These findings add support to the hypothesis that secretory vesicle-associated proteins are particularly important, but not the exclusive, targets of the autoimmune response in type 1 diabetes. Selective screening of the human proteome offers a useful approach for identifying new autoantigens in autoimmune diseases.

AB - The four major autoantigens (IA-2, IA-2 beta, GAD65 and insulin) of type 1 diabetes are all associated with dense core or synaptic vesicles. This raised the possibility that other secretory vesicle-associated proteins might be targets of the autoimmune response in type 1 diabetes. To test this hypothesis 56 proteins, two-thirds of which are associated with secretory vesicles, were prepared by in vitro transcription/translation and screened for autoantibodies by liquid phase radioimmunoprecipitation. Two secretory vesicle-associated proteins, VAMP2 and NPY, were identified as new minor autoantigens with 21% and 9%, respectively, of 200 type 1 diabetes sera reacting positively. These findings add support to the hypothesis that secretory vesicle-associated proteins are particularly important, but not the exclusive, targets of the autoimmune response in type 1 diabetes. Selective screening of the human proteome offers a useful approach for identifying new autoantigens in autoimmune diseases.

KW - type 1 diabetes

KW - secretory vesicles

KW - proteome

KW - phosphatase

KW - protein tyrosine

KW - IA-2

KW - GAD65

KW - autoantibodies

KW - autoantigens

U2 - 10.1016/j.clim.2008.01.018

DO - 10.1016/j.clim.2008.01.018

M3 - Article

VL - 127

SP - 366

EP - 374

JO - Clinical Immunology

T2 - Clinical Immunology

JF - Clinical Immunology

SN - 1521-6616

IS - 3

ER -