Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans

Research output: Contribution to journalArticle

Abstract

The BhMIR32 xyn11A gene, encoding an extracellular endoxylanase of potential interest in bio-bleaching applications, was amplified from Bacillus halodurans MIR32 genomic DNA. The protein encoded is an endo-1,4-beta-xylanase belonging to family 11 of glycosyl hydrolases. Its nucleotide sequence was analysed and the mature peptide was subcloned into pET22b(+) expression vector. The enzyme was over-expressed in a high density Escherichia coli culture as a soluble and active protein, and purified in a single step by immobilised metal ion affinity chromatography with a specific activity of 3073 IU mg(-1).

Details

Authors
  • Alejandra Martinez
  • Osvaldo Delgado
  • M D Baigori
  • F Sineriz
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Industrial Biotechnology

Keywords

  • purification, Bacillus halodurans MIR32, over-expression, sequence, xylanase
Original languageEnglish
Pages (from-to)545-550
JournalBiotechnology Letters
Volume27
Issue number8
Publication statusPublished - 2005
Publication categoryResearch
Peer-reviewedYes