Serum exchange and use of dilutions have improved precision of measurement of islet cell antibodies

Research output: Contribution to journalArticle

Abstract

In an attempt to improve the diagnostic value of measuring antibodies to islet cell cytoplasmic antigen, coded sera were distributed to 38 laboratories and results were returned for analysis. Comparison between laboratories revealed that results for some individual sera differed by up nine doubling dilutions and even within laboratories duplicate samples could differ by six doubling dilutions. By including dilutions of sera it was possible to draw a standard curve for each laboratory and this revealed major variations in shape, slope and intercept. However, using each laboratory's standard curve and converting results to units, a substantial improvement was obtained. The approach described improves standardisation and will permit laboratories to identify poor precision and/or any systematic change in assay performance.

Details

Authors
External organisations
  • Royal Perth Hospital
  • Hagedorn Research Institute
  • University of Western Australia, Crawley
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Clinical Laboratory Medicine

Keywords

  • Insulin-dependent diabetes mellitus, Islet cell antibody, Standards
Original languageEnglish
Pages (from-to)83-88
Number of pages6
JournalJournal of Immunological Methods
Volume106
Issue number1
Publication statusPublished - 1988 Jan 21
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes