Shape and Phase Transitions in a PEGylated Phospholipid System

Research output: Contribution to journalArticle

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Shape and Phase Transitions in a PEGylated Phospholipid System. / Viitala, Lauri; Pajari, Saija; Gentile, Luigi; Määttä, Jukka; Gubitosi, Marta; Deska, Jan; Sammalkorpi, Maria; Olsson, Ulf; Murtomäki, Lasse.

In: Langmuir, Vol. 35, No. 11, 2019, p. 3999–4010.

Research output: Contribution to journalArticle

Harvard

Viitala, L, Pajari, S, Gentile, L, Määttä, J, Gubitosi, M, Deska, J, Sammalkorpi, M, Olsson, U & Murtomäki, L 2019, 'Shape and Phase Transitions in a PEGylated Phospholipid System', Langmuir, vol. 35, no. 11, pp. 3999–4010. https://doi.org/10.1021/acs.langmuir.8b03829

APA

Viitala, L., Pajari, S., Gentile, L., Määttä, J., Gubitosi, M., Deska, J., ... Murtomäki, L. (2019). Shape and Phase Transitions in a PEGylated Phospholipid System. Langmuir, 35(11), 3999–4010. https://doi.org/10.1021/acs.langmuir.8b03829

CBE

Viitala L, Pajari S, Gentile L, Määttä J, Gubitosi M, Deska J, Sammalkorpi M, Olsson U, Murtomäki L. 2019. Shape and Phase Transitions in a PEGylated Phospholipid System. Langmuir. 35(11):3999–4010. https://doi.org/10.1021/acs.langmuir.8b03829

MLA

Vancouver

Viitala L, Pajari S, Gentile L, Määttä J, Gubitosi M, Deska J et al. Shape and Phase Transitions in a PEGylated Phospholipid System. Langmuir. 2019;35(11):3999–4010. https://doi.org/10.1021/acs.langmuir.8b03829

Author

Viitala, Lauri ; Pajari, Saija ; Gentile, Luigi ; Määttä, Jukka ; Gubitosi, Marta ; Deska, Jan ; Sammalkorpi, Maria ; Olsson, Ulf ; Murtomäki, Lasse. / Shape and Phase Transitions in a PEGylated Phospholipid System. In: Langmuir. 2019 ; Vol. 35, No. 11. pp. 3999–4010.

RIS

TY - JOUR

T1 - Shape and Phase Transitions in a PEGylated Phospholipid System

AU - Viitala, Lauri

AU - Pajari, Saija

AU - Gentile, Luigi

AU - Määttä, Jukka

AU - Gubitosi, Marta

AU - Deska, Jan

AU - Sammalkorpi, Maria

AU - Olsson, Ulf

AU - Murtomäki, Lasse

PY - 2019

Y1 - 2019

N2 - Poly(ethylene glycol) (PEG) polymers and PEG-conjugated lipids are widely used in bioengineering and drug transport applications. A PEG layer in a drug carrier increases hydrophilic repulsion, inhibits membrane fusion and serum opsonin interactions, and prolongs the storage and circulation time. It can also change the carrier shape and have an influence on many properties related to the content release of the carrier. In this paper, we focus on the physicochemical effects of PEGylation in the lipid bilayer. We introduce laurdanC as a fluorophore for shape recognition and phase transition detection. Together with laurdanC, cryogenic transmission electron microscopy, differential scanning calorimetry, molecular dynamics simulations, and small-angle X-ray scattering/wide-angle X-ray scattering, we acquire information of the particle/bilayer morphology and phase behavior in systems containing 1,2-dipalmitoyl-sn-glycero-3-phosphocholine:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-PEG(2000) with different fractions. We find that PEGylation leads to two important and potentially usable features of the system. (1) Spherical vesicles present a window of elevated chain-melting temperatures and (2) lipid packing shape-controlled liposome-to-bicelle transition. The first finding is significant for targets requiring multiple release sequences and the second enables tuning the release by composition and the PEG polymer length. Besides drug delivery systems, the findings can be used in other smart soft materials with trigger-polymers as well.

AB - Poly(ethylene glycol) (PEG) polymers and PEG-conjugated lipids are widely used in bioengineering and drug transport applications. A PEG layer in a drug carrier increases hydrophilic repulsion, inhibits membrane fusion and serum opsonin interactions, and prolongs the storage and circulation time. It can also change the carrier shape and have an influence on many properties related to the content release of the carrier. In this paper, we focus on the physicochemical effects of PEGylation in the lipid bilayer. We introduce laurdanC as a fluorophore for shape recognition and phase transition detection. Together with laurdanC, cryogenic transmission electron microscopy, differential scanning calorimetry, molecular dynamics simulations, and small-angle X-ray scattering/wide-angle X-ray scattering, we acquire information of the particle/bilayer morphology and phase behavior in systems containing 1,2-dipalmitoyl-sn-glycero-3-phosphocholine:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-PEG(2000) with different fractions. We find that PEGylation leads to two important and potentially usable features of the system. (1) Spherical vesicles present a window of elevated chain-melting temperatures and (2) lipid packing shape-controlled liposome-to-bicelle transition. The first finding is significant for targets requiring multiple release sequences and the second enables tuning the release by composition and the PEG polymer length. Besides drug delivery systems, the findings can be used in other smart soft materials with trigger-polymers as well.

U2 - 10.1021/acs.langmuir.8b03829

DO - 10.1021/acs.langmuir.8b03829

M3 - Article

VL - 35

SP - 3999

EP - 4010

JO - Langmuir

T2 - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 11

ER -