Signal frequency studies of an environmental application of a 65 nm region ion sensitive field effect transistor sensor

Research output: Contribution to journalArticle


A rapid and sensitive novel type of bioelectronic Region Ion Sensitive Field Effect Transistor (RISFET) nanosensor was constructed on a chip with a 65 nm sensing electrode gap. The RISFET nanosensor was demonstrated for the environmental pesticide analysis of neurotoxic organocarbamate/carbofuran. The linear range for carbofuran analysis is ac signal frequency dependent, studied in the range (500 down-0.5 Hz, 50 mV(peak-peak) ac) and a bias voltage applied between the bottom capacitor plate and the electrodes. The signal current response is measured using a low-noise pico ammeter. The inhibition of acetylcholinesterase (AChE) by carbofuran was detectable in a logarithmic linear range (0.1-100nM) at 1.08 Hz, with a lower limit of detection of inhibition 0.1 nM with 10 min incubation time. The sensor is based on the principle of focusing charged reaction products with an electrical field in a region between the sensing electrodes. The current measurement by the sensor electrodes is correlated to the composition of the sample. The carbofuran detection is based on the ability to inhibit the enzyme AChE. The RISFET sensor chip is fabricated using conventional electron beam lithography. The encompassed sensor volume in the "nanocell" is in the attoliter range.


  • Klas Risveden
  • Sunil Bhand
  • Fredrik Pontén
  • Thomas Anden
  • Nils Calander
  • Magnus Willander
  • Bengt Danielsson
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology


  • carbofuran, nanosensor, bioelectronic, RISFET, conducting channel
Original languageEnglish
Pages (from-to)198-203
JournalSensors and Actuators B: Chemical
Issue number1
Publication statusPublished - 2007
Publication categoryResearch