Simultaneous saccharification and co-fermentation of glucose and xylose in steam-pretreated corn stover at high fiber content with Saccharomyces cerevisiae TMB3400

Research output: Contribution to journalArticle


The two main sugars in the agricultural by-product corn stover are glucose and xylose. Co-fermentation of glucose and xylose at high content of water-insoluble solids (WIS) without detoxification is a prerequisite to obtain high ethanol concentration and to reduce production costs. A recombinant strain of Saccharomyces cerevisiae, TMB3400, was used in simultaneous saccharification and fermentation (SSF) of whole pretreated slurry of corn stover at high WIS. TMB3400 co-fermented glucose and xylose with relatively high ethanol yields giving high final ethanol concentration. The ethanol productivity increased with increasing concentration of pretreatment hydrolysate in the yeast production medium and when SSF was performed in a fed-batch mode.


Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Industrial Biotechnology
  • Chemical Engineering


  • TMB3400, Recombinant Saccharomyces cerevisiae, Glucose and xylose co-fermentation, Steam pretreatment, SSF, Ethanol
Original languageEnglish
Pages (from-to)488-498
JournalJournal of Biotechnology
Issue number4
Publication statusPublished - 2006
Publication categoryResearch