Single cell analysis of the common lymphoid progenitor compartment reveals functional and molecular heterogeneity.

Research output: Contribution to journalArticle


In order to investigate molecular events involved in the regulation of lymphoid lineage commitment, we crossed lambda5 reporter transgenic mice to Rag1-GFP knock in mice. This allowed us to sub-fractionate common lymphoid progenitors (CLPs) and pre-pro-B (Fraction A) cells into lambda5(-)Rag1(low), lambda5(-)Rag1(high) and lambda5(+)Rag1(high) cells. Clonal in vitro differentiation analysis demonstrated that Rag1(low) cells gave rise to B/T and NK cells. Rag1(high) cells displayed reduced NK-cell potential with preserved capacity to generate B- and T-lineage cells, while the lambda5(+) cells were B-lineage restricted. Ebf1 and Pax5 expression was largely confined to the Rag1(high) populations. These cells also expressed a higher level of the surface protein LY6D, providing an additional tool for the analysis of early lymphoid development. These data suggest that the classical CLP compartment comprises a mixture of cells with relatively restricted lineage potentials, thus opening new possibilities to investigate early hematopoiesis.


Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Hematology
Original languageEnglish
Pages (from-to)2601-2609
Issue number13
Publication statusPublished - 2010
Publication categoryResearch

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hematopoietic Stem Cell Laboratory (013022012), Connective Tissue Biology (013230151), Immunology (013212020), Division of Clinical Genetics (013022003)

Related research output

Welinder, E., 2012, Section for Immunology, Lund University. 109 p.

Research output: ThesisDoctoral Thesis (compilation)

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