Skin morphology and layer identification using different STIM geometries

Research output: Contribution to journalArticle


The use of on-axis geometry in scanning transmission ion microscopy (STIM) has been widely used for thin biological sample structure identification. In this configuration, the lateral resolution is optimised so that micron or submicron beam spots are easily achieved even for classic microbeam lines. Off-axis STIM was more particularly employed for rapid imaging, and also (when associated to a scattering set-up) for normalising elemental contents obtained by other ion beam analysis techniques in organic thin samples. Due to the very small beam current required, on-axis STIM is a stand-alone technique. Off-axis STIM can be advantageous as it enables the simultaneous utilisation of PIXE and RBS techniques. In this paper, the STIM images obtained with an on-axis geometry, a standard off-axis geometry and a recently developed on-off geometry are presented and discussed. Data from skin samples are used for comparison purposes aiming at studying skin permeability to sunscreens. Skin is a stratified tissue and the precise identification of skin layers is needed to ascertain the penetration depth of the physical filter from the formulation. In addition to the intrinsic difference in image quality due to the beam resolution, the influence of the detector type, implanted silicon detector versus Si pin diode, is discussed on the basis of their energy resolution.. their resistance to beam damage as well as the effect


  • P Aguer
  • LC Alves
  • P Barberet
  • E Gontier
  • S Incerti
  • C Michelet-Habchi
  • Z Kertesz
  • AZ Kiss
  • P Moretto
  • Jan Pallon
  • T Pinheiro
  • JE Surleve-Bazeille
  • Z Szikszai
  • A Verissimo
  • MD Ynsa
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Subatomic Physics


  • beam damage, image quality, STIM
Original languageEnglish
Pages (from-to)292-299
JournalNuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms
Issue number1-4
Publication statusPublished - 2005
Publication categoryResearch

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Nuclear Physics (Faculty of Technology) (011013007)