Slalom encodes an adenosine 3'-phosphate 5'-phosphosulfate transporter essential for development in Drosophila.

Research output: Contribution to journalArticle

Abstract

Sulfation of all macromolecules entering the secretory pathway in higher organisms occurs in the Golgi and requires the high-energy sulfate donor adenosine 3'-phosphate 5'-phosphosulfate. Here we report the first molecular identification of a gene that encodes a transmembrane protein required to transport adenosine 3'-phosphate 5'-phosphosulfate from the cytosol into the Golgi lumen. Mutations in this gene, which we call slalom, display defects in Wg and Hh signaling, which are likely due to the lack of sulfation of glycos aminoglycans by the sulfotransferase sulfateless. Analysis of mosaic mutant ovaries shows that sll function is also essential for dorsal–ventral axis determination, suggesting that sll transports the sulfate donor required for sulfotransferase activity of the dorsal–ventral determinant pipe.

Details

Authors
  • Florian Lueders
  • Hiroaki Segawa
  • David Stein
  • Erica M. Selva
  • Norbert Perrimon
  • Salvatore J. Turco
  • Udo Häcker
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology

Keywords

  • sulfation, Pipe, glycosaminoglycan, Hedgehog, Wingless
Original languageEnglish
Pages (from-to)3635-3644
JournalEMBO Journal
Volume22
Issue number14
Publication statusPublished - 2003
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Developmental biology (LUR000007), Invertebral Developmental Biology, Udo Haecker's group (013212048)