Sodium dodecyl sulfate free gel electrophoresis/electroelution sorting for peptide fractionation

Research output: Contribution to journalArticle

Abstract

Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of sodium dodecyl sulfate polyacrylamide gel electrophoresis for protein fractionation and sodium dodecyl sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for sodium dodecyl sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.

Details

Authors
  • Yassel Ramos
  • Annia González
  • Patricia Sosa-Acosta
  • Yasset Perez-Riverol
  • Yairet García
  • Lila Castellanos-Serra
  • Jeovanis Gil
  • Aniel Sánchez
  • Luis J. González
  • Vladimir Besada
Organisations
External organisations
  • Center for Genetic Engineering and Biotechnology (CIGB)
  • Wellcome Trust Genome Campus
  • Skåne University Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology

Keywords

  • electrophoresis, peptide fractionation, proteomics
Original languageEnglish
JournalJournal of Separation Science
Publication statusE-pub ahead of print - 2019
Publication categoryResearch
Peer-reviewedYes