Standardization of Cystatin C: development of primary and secondary reference preparations.

Research output: Contribution to journalArticle

Abstract

A Primary Reference Preparation has been produced using pure, recombinant, Cystatin C in a solvent of 0.1 mol/L KCl. Dry mass determination of the Primary Reference Preparation resulted in a Cystatin C concentration of 5.20 g/L. Agarose-electrophoresis and SDS-electrophoresis, as well as N-terminal sequencing, verified the purity, homogeneity and identity of Cystatin C in the Primary Reference Preparation. For the Secondary Reference Preparation, a serum pool was collected and stabilized. A pilot batch was made to verify the selected procedure and spiking with the pure, recombinant Cystatin C. The final Secondary Reference Preparation is now produced (4468 vials) and ready for value assignment and further characterization.

Details

Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medicinal Chemistry
  • Pharmacology and Toxicology

Keywords

  • Primary Reference Preparation, Secondary Reference Preparation, standardization of Cystatin C, dry mass determination, Cystatin C
Original languageEnglish
Pages (from-to)67-70
JournalScandinavian Journal of Clinical & Laboratory Investigation
Volume68
Issue numbers241
Publication statusPublished - 2008
Publication categoryResearch
Peer-reviewedYes

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Research output: ThesisDoctoral Thesis (compilation)

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