Statistic estimation of cell compressibility based on acoustophoretic separation data

Research output: Contribution to journalArticle

Abstract

We present a new experimental method that measures the compressibility of phenotype-specific cell populations. This is done by performing statistical analysis of the cell counts from the outlets of an acoustophoresis chip as a function of the increasing actuator voltage (i.e. acoustic energy density) during acoustophoretic separation. The theoretical separation performance curve, henceforth, Side-Stream Recovery (SSR), vs the piezo-actuator voltage (V) is derived by moment analysis of a one-dimensional model of acoustophoresis separation, accounting for distributions of the cell or microparticle properties and the system parameters (hydrodynamics, radiation force, drag enhancement, and acoustic streaming). The acoustophoretic device is calibrated with polymer microbeads of known properties by fitting the experimental SSR with the theoretical SSR , in which the acoustic energy density is considered proportional to the squared voltage, i.e. Eac=αV2. The fitting parameter α for the calibration procedure is the device effectivity, reflecting the efficiency in performing acoustophoretic microparticle displacement. Once calibrated, the compressibility of unknown cells is estimated by fitting experimental SSR cell data points with the theoretical SSR curve. In this procedure, the microparticle compressibility is the fitting parameter. The method is applied to estimate the compressibility of a variety of cell populations showing its utility in terms of rapid analysis and need for minute sample amounts.

Details

Authors
Organisations
External organisations
  • University of Twente
  • Skåne University Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medical Laboratory and Measurements Technologies

Keywords

  • Acoustofluidics, Acoustophoresis, Dispersion, Measurements, Microparticle compressibility, Statistics
Original languageEnglish
Article number64
JournalMicrofluidics and Nanofluidics
Volume24
Issue number8
Publication statusPublished - 2020 Aug 1
Publication categoryResearch
Peer-reviewedYes