Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin

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Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin. / Nitsche, Julius; Josts, Inokentijs; Heidemann, Johannes; Mertens, Haydyn D; Maric, Selma; Moulin, Martine; Haertlein, Michael; Busch, Sebastian; Forsyth, V Trevor; Svergun, Dmitri I; Uetrecht, Charlotte; Tidow, Henning.

In: Communications Biology, Vol. 1, 2018, p. 206.

Research output: Contribution to journalArticle

Harvard

Nitsche, J, Josts, I, Heidemann, J, Mertens, HD, Maric, S, Moulin, M, Haertlein, M, Busch, S, Forsyth, VT, Svergun, DI, Uetrecht, C & Tidow, H 2018, 'Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin', Communications Biology, vol. 1, pp. 206. https://doi.org/10.1038/s42003-018-0203-7

APA

Nitsche, J., Josts, I., Heidemann, J., Mertens, H. D., Maric, S., Moulin, M., ... Tidow, H. (2018). Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin. Communications Biology, 1, 206. https://doi.org/10.1038/s42003-018-0203-7

CBE

Nitsche J, Josts I, Heidemann J, Mertens HD, Maric S, Moulin M, Haertlein M, Busch S, Forsyth VT, Svergun DI, Uetrecht C, Tidow H. 2018. Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin. Communications Biology. 1:206. https://doi.org/10.1038/s42003-018-0203-7

MLA

Vancouver

Author

Nitsche, Julius ; Josts, Inokentijs ; Heidemann, Johannes ; Mertens, Haydyn D ; Maric, Selma ; Moulin, Martine ; Haertlein, Michael ; Busch, Sebastian ; Forsyth, V Trevor ; Svergun, Dmitri I ; Uetrecht, Charlotte ; Tidow, Henning. / Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin. In: Communications Biology. 2018 ; Vol. 1. pp. 206.

RIS

TY - JOUR

T1 - Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin

AU - Nitsche, Julius

AU - Josts, Inokentijs

AU - Heidemann, Johannes

AU - Mertens, Haydyn D

AU - Maric, Selma

AU - Moulin, Martine

AU - Haertlein, Michael

AU - Busch, Sebastian

AU - Forsyth, V Trevor

AU - Svergun, Dmitri I

AU - Uetrecht, Charlotte

AU - Tidow, Henning

PY - 2018

Y1 - 2018

N2 - Plasma-membrane Ca2+-ATPases expel Ca2+ from the cytoplasm and are key regulators of Ca2+ homeostasis in eukaryotes. They are autoinhibited under low Ca2+ concentrations. Calmodulin (CaM)-binding to a unique regulatory domain releases the autoinhibition and activates the pump. However, the structural basis for this activation, including the overall structure of this calcium pump and its complex with calmodulin, is unknown. We previously determined the high-resolution structure of calmodulin in complex with the regulatory domain of the plasma-membrane Ca2+-ATPase ACA8 and revealed a bimodular mechanism of calcium control in eukaryotes. Here we show that activation of ACA8 by CaM involves large conformational changes. Combining advanced modeling of neutron scattering data acquired from stealth nanodiscs and native mass spectrometry with detailed dissection of binding constants, we present a structural model for the full-length ACA8 Ca2+ pump in its calmodulin-activated state illustrating a displacement of the regulatory domain from the core enzyme.

AB - Plasma-membrane Ca2+-ATPases expel Ca2+ from the cytoplasm and are key regulators of Ca2+ homeostasis in eukaryotes. They are autoinhibited under low Ca2+ concentrations. Calmodulin (CaM)-binding to a unique regulatory domain releases the autoinhibition and activates the pump. However, the structural basis for this activation, including the overall structure of this calcium pump and its complex with calmodulin, is unknown. We previously determined the high-resolution structure of calmodulin in complex with the regulatory domain of the plasma-membrane Ca2+-ATPase ACA8 and revealed a bimodular mechanism of calcium control in eukaryotes. Here we show that activation of ACA8 by CaM involves large conformational changes. Combining advanced modeling of neutron scattering data acquired from stealth nanodiscs and native mass spectrometry with detailed dissection of binding constants, we present a structural model for the full-length ACA8 Ca2+ pump in its calmodulin-activated state illustrating a displacement of the regulatory domain from the core enzyme.

U2 - 10.1038/s42003-018-0203-7

DO - 10.1038/s42003-018-0203-7

M3 - Article

VL - 1

SP - 206

JO - Communications Biology

JF - Communications Biology

SN - 2399-3642

ER -