Structure of an autoimmune T cell receptor complexed with class II peptide-MHC: Insights into MHC bias and antigen specificity

Research output: Contribution to journalArticle

Abstract

T cell receptor crossreactivity with different peptide ligands and biased recognition of MHC are coupled features of antigen recognition that are necessary for the T cell's diverse functional repertoire. In the crystal structure between an autoreactive, EAE T cell clone 172.10 and myelin basic protein (1-11) presented by class II MHC I-Au, recognition of the MHC is dominated by the Vβ domain of the TCR, which interacts with the MHC α chain in a manner suggestive of a germline-encoded TCR/MHC "anchor point." Strikingly, there are few specific contacts between the TCR CDR3 loops and the MBP peptide. We also find that over 1,000,000 different peptides derived from combinatorial libraries can activate 172.10, yet the TCR strongly prefers the native MBP contact residues. We suggest that while TCR scanning of pMHC may be degenerate due to the TCR germline bias for MHC, recognition of structurally distinct agonist peptides is not indicative of TCR promiscuity, but rather highly specific alternative solutions to TCR engagement.

Details

Authors
  • Jennifer Maynard
  • Karin Lindkvist
  • Dianne H. Wilson
  • Erin J. Adams
  • Sylvie E Blondelle
  • Marty J. Boulanger
  • Darcy B. Wilson
  • K. Christopher Garcia
External organisations
  • Stanford University
  • Torrey Pines Institute for Molecular Studies
Original languageEnglish
Pages (from-to)81-92
Number of pages12
JournalImmunity
Volume22
Issue number1
Publication statusPublished - 2005 Jan
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes