Studies on the interaction between hyaluronan and a rat colon cancer cell line

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Studies on the interaction between hyaluronan and a rat colon cancer cell line. / Samuelsson, C; Gustafson, S.

In: Glycoconjugate Journal, Vol. 15, No. 2, 02.1998, p. 169-75.

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TY - JOUR

T1 - Studies on the interaction between hyaluronan and a rat colon cancer cell line

AU - Samuelsson, C

AU - Gustafson, S

PY - 1998/2

Y1 - 1998/2

N2 - Binding studies with 125I-Tyr labelled hyaluronan (HA) on a cultured rat colon cancer cell line were performed to characterize the association of HA to tumour cells in vitro. Results show a specific and saturable binding (Kd=1.36 nM) which indicates the presence of an HA binding receptor on the tumour cells. There is a specific constant increase of cell-associated HA over time, which indicates that HA is specifically taken up by the cells through endocytosis. The binding of 125I-Tyr labelled HA was more effectively inhibited by unlabelled HA of high MW in relation to low MW species of the polysaccharide indicating that the receptor binds HA of high MW with greater affinity than low MW species. In competition experiments, the HA-binding could not be inhibited by other polysaccharides such as chondroitin sulphate and heparin. Nor could ligands for scavenger receptors and antibodies directed towards ICAM-1, CD 44 and RHAMM (Receptor for HA Mediated Motility) significantly inhibit the association of HA to tumour cells.

AB - Binding studies with 125I-Tyr labelled hyaluronan (HA) on a cultured rat colon cancer cell line were performed to characterize the association of HA to tumour cells in vitro. Results show a specific and saturable binding (Kd=1.36 nM) which indicates the presence of an HA binding receptor on the tumour cells. There is a specific constant increase of cell-associated HA over time, which indicates that HA is specifically taken up by the cells through endocytosis. The binding of 125I-Tyr labelled HA was more effectively inhibited by unlabelled HA of high MW in relation to low MW species of the polysaccharide indicating that the receptor binds HA of high MW with greater affinity than low MW species. In competition experiments, the HA-binding could not be inhibited by other polysaccharides such as chondroitin sulphate and heparin. Nor could ligands for scavenger receptors and antibodies directed towards ICAM-1, CD 44 and RHAMM (Receptor for HA Mediated Motility) significantly inhibit the association of HA to tumour cells.

KW - Animals

KW - Antibodies/pharmacology

KW - Chondroitin Sulfates/pharmacology

KW - Colonic Neoplasms/drug therapy

KW - Dextran Sulfate/pharmacology

KW - Endothelium/cytology

KW - Extracellular Matrix Proteins/immunology

KW - Hyaluronan Receptors/immunology

KW - Hyaluronic Acid/metabolism

KW - Intercellular Adhesion Molecule-1/immunology

KW - Liver/cytology

KW - Mice

KW - Rats

KW - Rats, Wistar

KW - Tumor Cells, Cultured

U2 - 10.1023/A:1006920323095

DO - 10.1023/A:1006920323095

M3 - Article

C2 - 9557877

VL - 15

SP - 169

EP - 175

JO - Glycoconjugate Journal

JF - Glycoconjugate Journal

SN - 1573-4986

IS - 2

ER -