Substrate-dependent production and some properties of a thermostable, α-galactosidase from Rhodothermus marinus
Research output: Contribution to journal › Article
α-Galactosidase activity in Rhodothermus marinus is dependent on the composition of the growth media. A maximum of 46 μU g-1 cell dry weight was obtained using minimal medium with galactooligo- or polysaccharides as single carbon source. An enzyme hydrolysing both high and low molecular weight galacto-saccharides was partly purified from the cell fractions. The molecular weight was 200 kDa (native) and 50 kDa (monomer). It was optimally active at 85°C, with a half-life of 2 h at 75°C, and had a broad pH range (4-8).
|Research areas and keywords||
Subject classification (UKÄ) – MANDATORY
|Number of pages||7|
|Publication status||Published - 2000 Apr 1|