Substrate-dependent production and some properties of a thermostable, α-galactosidase from Rhodothermus marinus

Research output: Contribution to journalArticle


α-Galactosidase activity in Rhodothermus marinus is dependent on the composition of the growth media. A maximum of 46 μU g-1 cell dry weight was obtained using minimal medium with galactooligo- or polysaccharides as single carbon source. An enzyme hydrolysing both high and low molecular weight galacto-saccharides was partly purified from the cell fractions. The molecular weight was 200 kDa (native) and 50 kDa (monomer). It was optimally active at 85°C, with a half-life of 2 h at 75°C, and had a broad pH range (4-8).


External organisations
  • University of Kalmar
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biocatalysis and Enzyme Technology


  • Galactosides, Glycosyl hydrolase, Thermophilic
Original languageEnglish
Pages (from-to)663-669
Number of pages7
JournalBiotechnology Letters
Issue number8
Publication statusPublished - 2000 Apr 1
Publication categoryResearch