Supercritical fluid extraction of a lignocellulosic hydrolysate of spruce for detoxification and to facilitate analysis of inhibitors

Research output: Contribution to journalArticle


This work describes a novel approach to detoxify lignocellulosic hydrolysates and facilitate the analysis of inhibitory compounds, namely supercritical fluid extraction (SFE). The efficiency of the fermentation of lignocellulosic dilute-acid hydrolysates depends upon the composition of the hydrolysate and the organism used. Furthermore, it has been shown that inhibitors in the hydrolysate reduce the fermentation yield. This knowledge has given rise to the need to identify and remove the inhibiting compounds. Sample clean-up or work-up steps, to provide a clean and concentrated sample for the analytical system, facilitate the characterization of inhibitors, or indeed any compound in the hydrolysates. Removal of inhibitors was performed with countercurrent flow supercritical fluid extraction of liquid hydrolysates. Three different groups of inhibitors (furan derivatives, phenolic compounds, and aliphatic acids) and sugars were subsequently analyzed in the hydrolysate, extracted hydrolysate, and extract. The effect of the SFE treatment was examined with respect to fermentability with Saccharomyces cerevisiae. Not only did the extraction provide a clean and concentrated sample (extract) for analysis, but also a hydrolysate with increased fermentability as well as lower concentrations of inhibitors such as phenolics and furan derivatives. (C) 2002 Wiley Periodicals, Inc.


  • Per Persson
  • S Larsson
  • Leif Jonsson
  • NO Nilvebrant
  • Björn Sivik
  • Florentina Munteanu
  • Lars Thörneby
  • Lo Gorton
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Analytical Chemistry
  • Food Engineering
  • Industrial Biotechnology


  • fermentation, supercritical fluid extraction (SFE), detoxification, lignocellulosic hydrolysates, inhibitors, softwood
Original languageEnglish
Pages (from-to)694-700
JournalBiotechnology and Bioengineering
Issue number6
Publication statusPublished - 2002
Publication categoryResearch

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Food Technology (011001017), Applied Microbiology (LTH) (011001021), Analytical Chemistry (S/LTH) (011001004), LTH School of Engineering in Helsingborg (011045002)