Superior neovascularization and muscle regeneration in ischemic skeletal muscles following VEGF gene transfer by rAAV1 pseudotyped vectors

Research output: Contribution to journalArticle

Abstract

Recombinant adeno-associated virus serotype 2 (rAAV2) vector has been widely employed for gene therapy. Recent progress suggests that the new serotypes of AAV showed a better performance than did AAV2 in normal tissues. Here, we evaluate the potential role of human vascular endothelial growth factor (VEGF) gene transfer using rAAV vector pseudotyped with serotype I capsid proteins (rAAV1) in the treatment of muscle ischemia. In ischemic skeletal muscles, the rAAV1-LacZ vector allowed higher level, broader distribution, and long-lasting gene expression compared with the rAAV2-LacZ vector. Muscle VEGF165 production following the rAAV1-VEGF165 vector injection was 5-10 times higher than that following the rAAV2-VEGF165 vector injection. VEGF165 production mediated by the rAAV1-VEGF165 vector stimulated a large set of neovascularization with relatively mature vascular structures and enhanced muscle regeneration in the ischemic skeletal muscles. Thus, the rAAV1-NEGF165 vector mediated gene transfer may be a therapeutic approach to peripheral vascular diseases.

Details

Authors
  • H Yan
  • YH Guo
  • P Zhang
  • LY Zu
  • XY Dong
  • L Chen
  • JW Tian
  • Xiaolong Fan
  • NP Wang
  • XB Wu
  • W Gao
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biological Sciences

Keywords

  • endothelial growth factor, vascular, gene delivery, serotype 1, adeno-associated virus, muscle, ischemia
Original languageEnglish
Pages (from-to)287-298
JournalBiochemical and Biophysical Research Communications
Volume336
Issue number1
Publication statusPublished - 2005
Publication categoryResearch
Peer-reviewedYes